稗草和莴苣种子休眠及萌发生理的研究.docx

目录目录摘 要 ................................................................................................................. iABSTRACT ........................................................................................................ iii第 1 章 前言 ....................................................................................................... 11.1 种子的休眠与萌发 ........................................................................................... 11.2 种子的结构对种子休眠与萌发的影响 ........................................................... 11.2.1 后熟和冷层积对种子休眠与萌发的影响 ............................................ 11.2.2 种皮对种子休眠与萌发的影响 ............................................................ 11.2.3 降解细胞壁的三种酶对种子休眠与萌发的影响 ................................ 21.3 温度对种子休眠与萌发的影响 ....................................................................... 21.4 激素对种子休眠与萌发的影响 ....................................................................... 21.5 硝酸盐对种子热抑制的影响 ........................................................................... 41.6 稗草与莴苣种子研究概述 ............................................................................... 41.7 本实验研究的目的及意义 ............................................................................... 5第 2 章稗草种子休眠的释放及萌发过程中b-甘露聚糖酶、b-甘露糖甘酶和a-半乳糖苷酶的酶活性变化 ........................................................................................... 62.1 材料 ................................................................................................................... 62.1.1 植物材料 ................................................................................................ 62.1.2 试剂 ........................................................................................................ 62.2 实验方法 ........................................................................................................... 62.3 结果与分析 ....................................................................................................... 92.3.1 稗草种子萌发对温度的响应 ................................................................ 92.3.2 GA、6-BA 和划伤对稗草种子萌发的影响 ....................................... 102.3.3 后熟对稗草种子萌发的影响 .............................................................. 102.3.4 冷层积对稗草种子萌发的影响 .......................................................... 112.3.5 稗草种子萌发过程中b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性变化 ................................................................................................................ 122.3.6 后熟对b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性的影响 .................................................................................................................................... 132.3.7 冷层积对b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性的影响 ................................................................................................................................ 132.4 讨论 ................................................................................................................. 142.4.1 稗草种子休眠与萌发的特性 .............................................................. 14第 I 页目录2.4.2 稗草种子萌发过程中b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性变化 ................................................................................................................ 142.4.3 后熟对b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性的影响 .................................................................................................................................... 152.4.4 冷层积对b-甘露聚糖酶、b-甘露糖苷酶和a-半乳糖苷酶的酶活性的影响 ................................................................................................................................ 152.5 小结 ................................................................................................................. 16第 3 章硝酸盐调控莴苣种子热抑制过程中 ABA 和 GA 生物合成及降解关键基因的表达 .............................................................................................................. 173.1 材料 ................................................................................................................. 173.1.1 植物材料 .............................................................................................. 173.1.2 试剂耗材 .............................................................................................. 173.2 实验方法 ......................................................................................................... 173.2.1 萌发实验 .............................................................................................. 173.2.2 莴苣种子热萌发的恢复实验 .............................................................. 183.2.3 硝酸盐对莴苣种子萌发的作用 ........................................................... 183.2.4 RNA 提取.............................................................................................. 183.2.5 cDNA 的合成........................................................................................ 183.2.6 实时定量 PCR....................................................................................... 19。