dna内切酶名称、位点序列及切点(便于查找)

1、22. The chemical tool of claim 21 wherein the restriction endonuclease for targeting DNA sequences is selected from the group consisting of:Sequence cut Enzyme Name SEQ ID NO:AAR/AATTY AcsI 53R/AATTY ApoI 53A/AGCTT HindIII 54AA/CGTT Psp140 6I 55AAT/ATT SspI 56ACA/CRYGT AflIII 57A/CCGGT AgeI 58A/CATGT BspLU11I 59ACCTGC(4,8) BspMI 60, 61ACTGG(1,611) BsrI 62, 63R/CCGGY BsrFI 64R/CCGGY Cfr10I 64A/CGT MaeII 65A/CGCGT MluI 66RCATG/Y NspI 67A/CCGGT PinAI 58A/CCWGGT SexAI 68A/CTAGT SpeI 69AGAGG/CCT AatI

2、 70AG/CT AluI 71A/GATCT BglII 72R/GATCY BstYI 73RG/GNCCY DraII 74AGC/GCT Eco47III 75RG/GNCCY EcoO109I 74RGCGC/Y HaeII 76RG/GWCCY PpuMI 77AGT/ACT ScaI 78AGG/CCT StuI 70R/GATCY XhoII 73ATAT/TAAT AseI 79AT/TAAT AsnI 79AT/CGAT BspDI 80AT/CGAT ClaI 80ATGCA/T NsiI 81ATTT/AAAT SwaI 82CACAGNNN/CTG AlwNI 83CAC/GTG BbrPI 84CACNNN/GTG DraIII 85YAC/GTR BsaAI 86C/AATTG MfeI 87C/AATTG MunI 87CA/TATG NdeI 88CATG/ NlaIII 89CMG/CKG NspBII 90CAC/GTG PmaCI 84CAC/GTG PmlI 84CAG/CTG PvuII 91CR/CCGGYG SgrAI 92(13,9)C

3、ATCC FokI 93, 94CCC/CGC AciI 95CC/TNAGG AocI 96/CCWGG ApyI 97C/CTAGG AvrII 98C/CTAGG BinI 98C/CNNGG BsaJI 99CCNNNNN/NNGG BsiYI 23CCNNNNN/NNGG BslI 23CCANNNNN/NTGG BssGI 27CC/WGG BstNI 100CCANNNNN/NTGG BstXI 27CC/TNAGG Bsu36I 96C/CRYGG DsaI 101C/YCGRG AvaI 102CCTNN/NNNAGG EcoNI 32/CCWGG EcoRII 97C/CGG HpaII 103CCGC/GG KspI 104CCTC(7,6) MnlI 105, 106C/CGG MspI 103CC/TNAGG MstII 96CC/WGG MvaI 100CC/SGG NciI 107C/CATGG NcoI 108CMG/CKG NspBII 90CCANNNN/NTGG PflMI 44CCGC/GG SacII 104CC/TNAGG SauI 96CC

4、/NGG ScrFI 109CCC/GGG SmaI 110CCTGCA/GG Sse8387I 111CCGC/GG SstII 104C/CWWGG StyI 112CCANNNN/NTGG Van91I 44CCANNNNN/NNNNTGG XcmI 50C/CCGGG XmaI 113C/CCGGG XmaCI 113(1,611)CCAGT BsrI 114, 115CG(10,12)CGANNNNNNTGC(12,10) BcgI 116, 117CGRY/CG BsiEI 118C/GTACG BsiWI 119CG/CG BstUI 120C/GGCCG EagI 121Y/GGCCR CfrI 122Y/GGCCR EaeI 122C/GGCCG EclXI 121CGTCTC(1,5) Esp3I 123, 124CG/CG FnuDII 120CG/CG MvnI 120CGAT/CG PvuI 125CG/GWCCG RsrII 126CR/CCGGYG SgrAI 92CG/CG ThaI 120C/GGCCG XmaIII 121CTC/TTAAG AflI

5、I 127C/TAG BfaI 128C/TTAAG BfrI 127CTGGAG(16,14) BpmI 129, 130C/TNAG DdeI 131CTCTTC(1,4) EarI 132, 133C/YCGRG AvaI 102CTGAAG(16,14) Eco57I 134, 135CTGGAG(16,14) GsuI 129, 130C/TAG MaeI 128C/TCGAG PaeR7I 136CTGCA/G PstI 137C/TAG RmaI 128C/TRYAG SfcI 138C/TCGAG XhoI 136(14,16)CTCCAG BpmI 139, 140(14,16)CTGCAC BsgI 141, 142(14,16)CTTCAG Eco57I 143, 144(14,16)CTCCAG GsuI 139, 140GAGACGT/C AatII 145GACN/NNGTC AspI 146GAANN/NNTTC Asp700 8GACNNN/NNGTC AspEI 9GAAGAC(2,6) BbsI 147, 148GAAGAC(2,6) BpuAI 1

6、47, 148GATNN/NNATC BsaBI 20GAATGC(1,611) BsmI 149, 150GA/TC DpnI only if 151G-Me/GATC DpnII 151GACNNNN/NNGTC DrdI 28GACNNN/NNGTC Eam1105I 9GAG/CTC Ecl136II 152R/AATTY AcsI 53GR/CGYC AcyI 153GR/CGYC AhaII 153R/AATTY ApoI 53GWGCW/C AspHI 154GRGCY/C BanII 155GDGCH/C BmyI 156GR/CGYC BsaHI 153GDGCH/C Bsp1286I 156G/AATTC EcoRI 157GAT/ATC EcoRV 158GACGC(5,10) HgaI 159, 160,GWGCW/C HgiAI 154G/ANTC HinfI 161GATNN/NNATC MamI 20/GATC MboI 151GAAGA(8,7) MboII 162, 163/GATC NdeII 151GDGCH/C NspII 156GAGTC(4,

7、5) PleI 164, 165GAGCT/C SacI 166/GATC Sau3AI 151GAGCT/C SstI 166G/AWTC TfiI 167GACN/NNGTC Tth111I 146GAANN/NNTTC XmnI 8(9,5)GATGC SfaNI 168, 169(5,4)GATCC AlwI 170, 171(5,1)GAGACC BsaI 172, 173(5,1)GAGAC BsmAI 174, 175(4,1)GAAGAG EarI 176, 177(5,1)GAGACG Esp3I 178, 179(6,7)GAGG MnlI 180, 181(5,4)GACTC PleI 182, 183GCGCAGC(8,12) BbvI 184, 185GCCNNNN/NGGC BglI 15GC/TNAGC Bpu1102I 186G/CGCGC BsePI 187G/CGCGC BssHII 187GC/TNAGC CelII 186GCG/C CfoI 188R/CCGGY BsrFI 64R/CCGGY Cfr10I 64GC/TNAGC EspI 18

8、6GC/NGC Fnu4HI 189GCG/C HhaI 188G/CGC HinPI 188GC/NGC ItaI 189GCC/GGC NaeI 190G/CCGGC NgoMI 191G/CTAGC NheI 192GC/GGCCGC NotI 193RCATG/Y NspI 67GCATC(5,9) SfaNI 194, 195GCATG/C SphI 196GCCC/GGGC SrfI 197G/CGG AciI 198(12,8)GCTGC BbvI 199, 200(10,12)GCANNNNNNTCG(12,10) BcgI 201, 202(1,1)GCATTC BsmI 203, 204(8,4)GCAGGT BspMI 205, 206(10,5)GCGTC HgaI 207, 208GGG/GTACC Acc65I 209G/GWCC AflI 210GGATC(4,5) AlwI 211, 212GGGCC/C ApaI 213GG/CGCGCC AscI 214G/GTACC Asp718 209G/GWCC AvaII 210G/GATCC BamHI 2

9、15G/GYRCC BanI 216GGTCTC(1,5) BsaI 217, 218G/GTNACC BstEII 219R/GATCY BstYI 73GR/CGYC AcyI 153GR/CGYC AhaII 153GRGCY/C BanII 155GDGCH/C BmyI 156GR/CGYC BsaHI 153GDGCH/C Bsp1286I 156RG/GNCCY DraII 74RG/GNCCY EcoO109I 74GGATG(9,13) FokI 220, 221GGCCGG/CC FseI 222RGCGC/Y HaeII 76GG/CC HaeIII 223GGTGA(8,7) HphI 224, 225G/GCGCC KasI 226GGTAC/C KpnI 227GG/CGCC NarI 228GGN/NCC NlaIV 229GDGCH/C NspII 156RG/GWCCY PpuMI 77G/GNCC Sau96I 230GGCCNNNN/NGGCC SfiI 49R/GATCY XhoII 73GTGT/MKAC AccI 231G/TGCAC Alw44I 232G/TGCAC ApaLI 232GTGCAG(16,14) BsgI 233, 234GTCTC(1,5) BsmAI 235, 236GTA/TAC Bst1107I 237GWGCW/C AspHI 154GDGCH/C BmyI 156GDGCH/C Bsp1286I 156GWGCW/C HgiAI 154GTY/RAC HincII 238GTY/RAC HindII 238GTT/AAC HpaI 239/GTNAC MaeIII 240GDGCH/C NspII 156GTTT/AAAC PmeI 241GT/AC RsaI 242G/TCGAC SalI 243G/TGCAC SnoI 232(6,2)GTCTTC BbsI 244, 245(6,2)GTCTTC BpuAI 244, 245TAYAC/GTR BsaAI 86TAC/GTA SnaBI 246TCT/CCGGA AccII 247T/CCGGA BseAI 247T/CCGGA BspEI 247T/CA

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