留学生essay写作—确定细菌生长
4页1、http:/ 留学生论文专业定制代写网站留学生essay写作确定细菌生长Introduction:This experiment was about isolating a single bacterial colony from a soil sample and identifying which genus it belongs in.This is especially important for functions such as agriculture,as knowing whether a specific soil has a high concentration of nutrient producing bacterium is essential to being able to harvest good crops.Another key component of soil bacterium,is that they tend to be high in nutrient recycling organisms.As well there tends to
2、be a high number of varying phylum in soil bacterium that was previously unknown,as in 2003 Joseph et al.managed to isolate 350 different bacterium which were assigned into 9 different phyla.As well approximately 27%of the isolated bacterium belonged to unnamed families,and were located in very poorly studied phyla.(Joseph et al.2003)Methods:The experiment began by subculturing a bacterial colony identified from a myriad of soil bacterium isolated in an agar plate.Then this bacterial colony was
3、observed through a microscope,as well as tested for whether it was gram positive or gram negative.Then the bacterium was re-cultured into varying solutions to test for specific nutrient use.First it was subcultured onto an agar plate rich in starch,incubated,and tested for starch hydrolysis via the use of lugols iodine,to see if there was starch remaining in the area of the bacterial colony.Then a deep rich in sulfur was inoculated with the bacterium,and observed for whether motility was display
4、ed,or whether hydrogen sulphide was produced.Then the bacterium was inoculated in a peptone broth,to test for production of ammonia,through the addition of Nesslers Reagent,an ammonium sulphate broth and a nitrite broth,to test for the ability to nitrify compounds using Nesslers reagent;Trommdorfs Reagent;diphenylamine;and Sulfuric acid,and a nitrate broth to test for the ability to denitrify compounds using indicated reagents.Then the bacterium was placed into a thioglycollate medium to test th
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