生物文献汇报
13页1、Target and non-target toxicity of botanical insecticide derived from Couroupita guianensis L. flower against generalist herbivore, Spodoptera litura Fab. and an earthworm, Eisenia foetida Savigny,XXX 2018.9,Introduction,Plants are the biochemical factories in nature able to produce a complex mixture of chemical compounds. C. guianensis, the flowers are used in medicine, the extracts have antifungal, antibiotic, antiseptic, analgesic properties and larvicidal activity. S. litura has hundred and f
2、ifty host types and settled resistance ranged from 100 to 2700 fold against pyrethroids. So it is urgent to find new ways to control S. litura. Earthworms are subtle indicators of soil quality, acting as decomposers in improving soil structure.,Content,Investigation of active compounds through gas chromatographic techniques. Assessment of the dose dependent toxicity of active compounds against S. litura. Measurement of the behavioral response of S. litura treated with active compounds of C. guia
3、nensis. Estimation of bioefficacy of active fractions on nutritional physiology of S. litura and effects of active fractions on gut histological alterations in the S. litura. Ecotoxicological evaluation of active fraction and chemical pesticides against E.fetida.,Methods and Result,Investigation of active compounds,Plant collection(fine particle size) Crude extract preparation(hexane, chloroform, ethyl acetate and methanol) Chromatographic analysis(F1, F2, F3, F4, F5 and F6 ) GCMS analysis(Fig.
4、1B. Table 1) FT-IR and HPLC analysis(Fig. 1C),GCMS FT-IR and HPLC analysis,Mortality bioassays,Bioassays were implemented on second, third and fourth larval instars of S. litura using concentrations of 100, 200, 400 and 800 ppm of fraction F6.,Developmental study,Developmental studies were executed with second instar larvae. Fresh leaves of castor treated with 50, 100, 150 and 200 ppm of fraction F6 and reared on larvae. Every 24 h, larval weight was recorded (Fig. 5). The duration of larval and
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