生物发光技术及其应用
70页1、Confidential and Proprietary. Not for Further Disclosure.Confidential and Proprietary. Not for Further Disclosure.Promega Founded 1978 Over 50 distributors in Europe, Asia, Africa and the Americas 1,400+ employees worldwide ISO 13485 certified Manufacturing in US, Korea and Shanghai Over 3,000 products for life science research and applied science including drug discovery Promega BioSystems Sunnyvale, Inc., CAPromega BioSciences, San Luis Obispo, CAPromega Headquarters and Manufacturing Madison,
2、 WisconsinPromega BioSystems, Seoul, KoreaShanghai Promega, ChinaConfidential and Proprietary. Not for Further Disclosure.Confidential and Proprietary. Not for Further Disclosure.Promega Provides Outstanding Life Science Research ToolsSystems BiologyProteomicsGenomicsCellomicsDNARNAProteinCellTissue Cellular Analysis Protein Protein Interaction Drug Screening Research Tools Little Animal Imaging Cell-Free Protein Expression DNA , RNA Purification and Analysis Epigenetics Research Tool2010, Prome
3、ga Corporation. Confidential and Proprietary. Not for Further Disclosure.段文娟 Senior Field Application Specialist 萤光素酶报告基因技术及其应用Confidential and Proprietary. Not for Further Disclosure.Confidential and Proprietary. Not for Further Disclosure.Content-目录目录1.报告基因技术简介报告基因技术简介2.萤光素酶报告基因技术应用3.萤光素酶报告基因技术检测法Confidential and Proprietary. Not for Further Disclosure.Confidential and Proprietary. Not for Further Disclosure.5Reporter Gene 报告基因的产生报告基因的产生PromoterExon 12345Enhancertranscription start siteEnhance
4、rReporter GenePromoter优势优势: 报告基因通常是容易检测的蛋白 报告基因可以通过生物发光、荧光、比色或放射的方法进行检测 采用酶类做为报告基因,具有放大信号的作用Reporter 问题问题:下游蛋白难以检测、方法繁琐Confidential and Proprietary. Not for Further Disclosure.Confidential and Proprietary. Not for Further Disclosure.Absorbance 物质的结构不同吸光波长也不同 物质对光的吸收与其浓度成正比 灵敏度有限灵敏度有限Four Methods For Assay 四种检测方法学四种检测方法学AbsorbanceAbsorbanceRadiationRadiationFluorescenceFluorescenceBioluminescenceBioluminescenceFluorescence Excitation from a photonBioluminescence Excitation from a chemical Reactio
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