中草药添加对大黄鱼基因表达的影响
15页1、摘 要谷胱甘肽过氧化物酶(GSH-Px)是机体内重要的氧化酶类之一。大黄鱼是我国重要的海水经济鱼类,也是宁德市的特色产业之一。本实验通过在大黄鱼饲料中添加不同比例的中草药复方(太子参巴戟当归枸杞茯苓白术),饲喂30天后,以脾脏组织为实验材料,采用实时荧光定量PCR技术分析饲喂中草药后GSH-Px基因的相对表达变化。结果显示:与对照组相比,各实验组中该基因的表达均出现了不同程度的下降(E5组除外),E3组的表达量下降最多(43%),其次是E1组(31%),E5组的表达量比对照组升高19%,但是各组间均无显著性差异(P大于0.05)。本研究结果为中草药作为饵料添加剂在大黄鱼饲料中的应用提供了基础数据,也为中草药作为鱼类免疫增强剂提供了前期研究基础。关键词:大黄鱼;基因表达;GSH-PxAbstractGlutathione peroxidase (GSH-Px) is one of the most important peroxidase enzymes in the body, which has an important effect on the innate immunity o
2、f fish. Larimichthys crocea is one of the most important economic fish in China and sea water is also one of the characteristic industry in Ningde. In this study, adding different proportion of Chinese herbal medicine compound were added in the feed of L. crocea (radix pseudostellariae, ba ji, angelica, medlar, poria cocos, atractylodes and so on). After feeding after 30 days, the spleen was isolated and extracted for RNA. The real-time fluorescent quantitative PCR was used to analyze the relati
3、ve expression of GSH in different groups. The results showed that compared with the control group, the gene expression in each experiment group were decreased (with the exception of E5 group), and the expression of E3 group decreased most (43%), followed by E1 group (31%), the expression of E5 group amount higher than the control group was 19%, but there was no significant difference between groups (P is greater than 0.05). The results of this study was to Chinese herbal medicine as bait additiv
4、es provide basic data for the application in the Larimichthys crocea feed, also provides the Chinese herbal medicine as a fish immune enhancer prophase research foundation.Key words: Larimichthys crocea; Gene expression; GSH-Px目录1 引言11.1大黄鱼11.2中草药及太子参主要简介11.3 GSH-Px基因22 材料和方法32.1 材料药品及仪器32.1.1原料鱼处理32.1.2试剂42.2 方法42.2.1总RNA提取42.2.2 RNA凝胶电泳42.2.3 RNA定量分析52.2.4 RNA反转录52.2.5 SYBRGreen实时荧光定量PCR53 实验结果及分析63.1 RNA的质量检测63.2熔解曲线分析73.3基因表达分析74 讨 论84.1 中草药添加相关应用与前景84.2 中草药添加对GSH-Px基因表达的影响84.3 总结9参 考 文 献10致
《中草药添加对大黄鱼基因表达的影响》由会员汽***分享,可在线阅读,更多相关《中草药添加对大黄鱼基因表达的影响》请在金锄头文库上搜索。
福建师范大学21秋《人力资源管理》概论在线作业三答案参考69
教学实习报告合集15篇
2023年个人述职报告开头和结尾
项目_数据库设计说明书
初三相似三角形讲义全
重点郴州市发改系统政策业务知识抢答赛题库
九年级下学期化学教师工作总结(6篇).doc
全国统一建筑工程预算工程量计算规则
科来网络分析系统50常见协议解码详解
急诊科年度工作计划标准范文(四篇).doc
2023-2024年学校工作计划(3篇).doc
2013年安徽省中考语文模拟试卷
未来五年幼师工资待遇
大学生万圣节互动游戏舞会活动方案
英语网络统考交际用语答题技巧
以评促教评价教学学习心得
《了解叙述的顺序》教案
上海自贸区金融改革..
校园交流活动策划方案
泡菜调料配方
2023-06-18 20页
2022-08-09 19页
2022-12-04 5页
2023-02-12 3页
2023-11-23 18页
2023-10-05 19页
2024-02-01 54页
2022-10-05 146页
2022-08-15 5页
2023-10-21 6页