神府煤的微生物转化作用研究.pdf

西安科技大学 硕士学位论文 神府煤的微生物转化作用研究 姓名：曾梅 申请学位级别：硕士 专业：矿物加工工程 指导教师：周安宁 @ 论文题目：神府煤的微生物转化作用研究 专 业：矿物加工工程 硕 士 生：曾 梅 （签名） 导 师：周安宁 （签名） 摘 要 煤炭作为一种宝贵的不可再生资源，如何使煤结构中芳环、杂环，以及萜类和蒎类 化合物得到充分有效利用，是目前煤炭科学与转化技术领域所关注的关键问题生物催 化技术可以高效地将反应底物转化为目标产物，并且对底物具有高度的立体选择性本 文采用生物转化方法，研究混合菌种和共基质对煤生物转化的影响，为煤生物定向转化 及溶煤机理提供理论依据 以光催化氧化 6h 神府煤（6UV-O-C）和光催化氧化 6h 神府煤碱提残煤（I-UV-C） 为研究对象，采用从神府洗煤废水中优选出的粗壮串珠霉（XK-a） 、黄绿青霉（XK-b） 和黄杆菌属 （XK-c） 为实验菌种对煤样进行生物转化 研究了菌种混合方式对6UV-O-C 、 I-UV-C 及腐植酸生物转化的影响 结果表明， 混合菌种转化煤及腐植酸效果没有单一菌 种好。

XK-a，XK-b 和 XK-c 组成的混合菌种对 6UV-O-C 的转化率均高于对 I-UV-C 的 转化率；XK-a 和 XK-b 混合转化腐植酸的总酸性官能团含量最高为 7.55mmol/g，而单 一菌种转化腐植酸的最高总酸性官能团含量分别是：XK-a 为 31.13mmol/g，XK-b 为 27.96mmol/g，XK-c 为 28.20mmol/gXK-a，XK-b 和 XK-c 之间属于竞争关系，不适合 混合培养 在无机盐培养基中加入愈创木酚、葡萄糖、苯酚和藜芦醇作为共基质，研究共基质 浓度对 XK-a 转化 I-UV-C 的影响结果表明，四种共基质均对生物溶煤有促进作用愈 创木酚、葡萄糖、苯酚、藜芦醇加入量分别为 0.43mL/L，1.0g/L，0.5g/L，0.29 mL/L 时， XK-a 对 I-UV-C 的转化率最高，分别为 27.63%，26.81%，16.84%，10.91%愈创木酚 和葡萄糖共基质培养基中，XK-a、XK-b 和 XK-c 转化 I-UV-C 均获得了较高的转化率， 分别为：33.05%，27.12%，31.77% 采用FTIR和元素分析方法研究了XK-a转化I-UV-C的产物结构。

结果表明，其碱沉 淀产物含有羟基、醚键、R-OH、-CH3，-CH2、芳香环等官能团，与原煤相比，碱沉淀 物的H/C比和N/C均提高了 3~4 倍，共基质存在条件下，转化残煤的H/C比明显增加，而 N/C比下降转化残煤的热重分析表明，加入共基质后，转化残煤的热稳定性下降 对加入不同共基质 XK-a 转化 I-UV-C 的动力学进行研究，不同共基质中 XK-a 转化 I-UV 关 词：神府煤；生物转化；混合菌种；共基质 研究类型：应用研究 -C 符合 Avrami-Erofeev 方程推导的微生物表观溶煤动力学模型 键 Subject ：：Study on the Microbial Process of Shenfu Coal Specialty ：：Mineral Process Engineering Name ：：Zeng Mei （（Signature）） Instructor：：Zhou Anning （（Signature）） ABSTRACT Because coal is a valuable non-renewable resource, the key issue of coal science and conversion technology is how to effectively utilize structure units of aromatic, heterocyclic, terpenes and pinene in coal. The substrate can be efficiently converted to product by biological catalyst which has high stereo-selectivity to the substrate. On the base of biocatalysis technology, this paper using biological transformation studied the effect of mixed strains and cosubstrate on the biological dissolution of coal, providing a theoretical basis for the oriented bioconversion and the explanation of coal dissolution mechanism in the biological conversion. Base on Shenfu coal treated by photocatalytic oxidation for 6 h (6UV-O-C) and the alkaline extraction residue of 6UV-O-C (I-UV-C), biological conversion of coal samples were studied using bacteria such as Monilia crassa (XK- a), Penicillium citreo viride (XK-b) and Flavobacterium (XK-c) which selected from Shenfu coal-washing wastewater. The effect of hybrid methods of strains on the convention of 6UV-O-C, I-UV-C and humic acid were studied. The results showed that the conversion rates of coal and humic acid by mixed bacteria were worse than single bacteria. The bioconversion rate of 6UV-O-C is higher than I-UV-C, 6UV-O-C and I-UV-C were converted by the mixed strains were composed of XK-a, XK-b and XK-c. The highest total acidic group of humic acid residue converted by mixed strains (XK-a and XK-b) was 7.55 mmol/g, while converted by single strain of XK-a, XK-b and XK-c were 31.13 mmol/g, 27.96 mmol/g, 28.20 mmol/g, respectively. The strains used in this experiment competed with each other, so they were not suitable for mixed culture. Guaiacol, glucose, phenol and veratryl alcohol in different concentrations were added in inorganic salt medium to study the effect of cosubstrate concentration on the XK-a convention of I-UV-C. All four types of cosubstrate promoted the biological dissolution of coal. The XK-a convention rate of I-UV-C was obtained as 27.63 %, 26.81 %, 16.84 %, 10.91 % with the guaiacol, glucose, phenol, veratryl alcohol dosage 0.43 mL/L, 1.0 g/L, 0.5 g/L, 0.29 mL/L respectively. Larger conversion rate of I-UV-C using XK-a, XK-b, XK-c were got in the medium with presence of guaiacol and glucose, as 33.05 %, 27.12 %, 31.77 %, respectively. The conversion products structure was characerized by FTIR and element analysis. FTIR analysis showed that alkaline precipitation products from XK-a convention of I-UV-C contain functional groups such as hydroxy, ether, R-OH, -CH , -CH and aromatic ring. Element 32 analysis showed that H / C ratio and N / C ratio of alkali sediments were 3 to 4 times than those of coal, and H / C ratio of the residual coal from cosubstrate conversion was significantly higher than that in the inorganic salt medium residue, while the N / C ratio decrease. Thermal analysis showed that the thermal stability of residual from cosubstrate convention was worse than that in inorganic salt medium. The dynamics for the convention process of I-UV-C in different cosubstrate using XK-a was studied, and the results showed that the convention process of I-UV-C in different cosubstrate using XK-a was in line with apparent microbial solubilization dynamic model derived from the Avrami-Erofeev equation. Keywords : Shenfu coal Bioconversion Mixed bacteria Cosubstrate Thesis : Applid Research 符号一览表 符号一览表 符号 说明 SFC 神府煤 6UV-O-C 光氧化 6h 神府煤 I-UV-C 光氧化 6h 神府煤碱提。