细胞培养对应培养基do.doc

常用细胞所用培养基配方CELL LINE CELL TYPE SPECIES TISSUE MEDIUM293 fibroblast human embryonic kidney MEM, 10% HI horse serum 3T6 fibroblast mouse embryo DMEM, 10% FBSDMEM, 10% FBSF-10, 15% horse serum andA549 epithelial human lung carcinoma F-12K, 10% FBS A9 fibroblast mouse connective tissue AtT・20 epithelial mouse pituitary tumor 2.5% FBSBALB/3T3 fibroblast mouse embryo DMEM, 10% FBSBHK-21 fibroblast hamster kidney GMEM, 10% FBS or MEM, 10% FBS and NEAABHL-100 epitheliall human breast McCoy5A3 10% FBSBT fibroblast bovine turbinate cells MEM, 10% FBS and NEAACaco-2 epithelial human colon adeno carcinoma MEM, 20% FBS and NEAAChang epithelial human liver BME, 10% calf serumCHO-K1 epithelial hamster ovary F-12, 10% FBSClone 9 epithelial rat liver F-12K, 10% FBSClone M-3 epithelial mouse melanoma F-10, 15% horse serum and2.5% FBSCOS-1COS-3COS-7CRFKfibroblastfibroblastfibroblastmonkey kidney DMEM, 10% FBSmonkey kidney DMEM, 10% FBSmonkey kidney DMEM, 10% FBSepithelial cat kidney MEM, 10% FBS and NEAACV-1 fibroblast monkey kidney MEM, 10% FBSD-17 epithelial dog osteosarcoma MEM, 10% FBS and NEAADaudi lymphoblast human blood from a lymphoma patient RPMI-1640,10% FBSGH1 epithelial rat pituitary tumor F-10, 15% horse serum and 2.5% FBS GH3 epithelial rat pituitary tumor F-10, 15% horse serum and 2.5% FBS H9 lymphoblast human T-cell lymphoma RPMI-1640,20% FBS HaK epithelial hamster kidney BME, 10% calf serum HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640, 10% FBSHeLa epithelial human cervix carcinoma MEM, 10% FBS and NEAA (in suspension, S-MEM)HEp-2 epithelial human larynx carcinoma MEM, 10% FBSHL-60 lymphoblast human promyeolocytic leukemia RPMI-1640,20% FBSHT-1080 epithelial human fibrosarcoma MEM, 10% HI FBS and NEAA HT-29 epithelial human colon adenocarcinoma McCoys 5A, 10% FBS HUVEC endothelial human umbilical cord F-12K, 10% FBS and heparinat 100 ug ml1-10 epithelial mouse testicular tumor F-10, 15% horse serum and 2.5% FBSIM-9 lymphoblast human marrow from myeloma patient RPMI-1640, 10% FBSJEG-2 epithelial human choriocarcinoma MEM, 10% FBSJensen fibroblast rat sarcoma McCoys 5A, 5% FBSJurkat lyphoblast human lymphoma RPMI-1640, 10% FBSK-562 lymphoblast human myelogenous leukemia RPMI-1640, 10% FBSKB epithelial human oral carcinoma MEM, 10% FBS and NEAAKG-1 myeloblast human marrow from erythroleukemia patient IMDM, 20% FBSL2 epithelial rat lung F-12K, 10%FBSL6 rat skeletal muscle myoblasts DMEM, 10% FBSLLC-WRC 256 epithelial rat carcinoma Medium 199, 5% horse serumMcCoy fibroblast mouse unknown MEM, 10% FBSMCF7 epithelial human breast adenocarcinoma MEM, 10% FBS NEAA, 10ug/ml insulinWEHI-3b macrophage-like mouse myelomonocyte leukemia DMEM, 10% FBSWI-38 epithelial human embryonic lung BME, 10% FBSWISH epithelial human amnion BME, 10% FBSWS1 human embryonic skin MEM, 10% FBS and NEAAXC epithelial rat sarcoma MEM, 10% FBS and NEAAY~1 epi thel ial mouse tumor of adrenal F-10, 15% horse serum and 2. 5% FBS细胞培养基的应用选择选择培养基没有--定的标准，有几点建议可供参考：(1)建立某种细胞株所用的培养基应该是培养这种细胞首选的培养基。

可以查阅参考文献，或在购买细胞株时咨询⑵ 其它实验室惯用的培养基不妨一试，许多培养基可以适合多种细胞⑶根据细胞株的特点、实验的需要来选择培养基如小鼠细胞株多选RPMI1640 o(4)用多种培养基培养目的细胞，观察其生长状态，可以用生长曲线、集 落形成率等指标判断，根据实验结果选择最佳培养基，这是最客观的方法， 但比较繁琐细胞系细胞类型种组织培养基293 清成纤维细胞人胚胎肾MEM, 10%热灭活马血IMR-90NEAA成纤维细胞人肺MEM, 10%胎牛血清和W1-38 胎牛血清成纤维细胞人胚胎肺,3月妊娠 MEM, 10%A549上皮细胞人肺癌F-12K, 10%胎牛血清A431上皮型人表皮细胞癌DMEM, 10%胎牛血清BIH-100上皮细胞人乳房McCoy5A, 10% 胎牛血清BeWo上皮滋养层人绒毛癌F-12K, 15%胎牛血清Caco-2上皮细胞人结肠腺癌MEM, 10%胎牛血清和 NEAAChang上皮细胞人肝脏BME, 10%小牛血清HOT-15上皮细胞人结肠直肠腺癌 RPMI-1640,10%胎牛血清HeLa上皮细胞人子宫颈癌MEM, 10%胎牛血清和 NEAA(in1 suspension,S-MEM)IIEp-G2上皮细胞人肝细胞癌MEM, 10%胎牛血清和 NEAAHEp-2上皮细胞人喉癌MEM, 10%胎牛血清；或RPMI-1640,10%胎牛血清HT-1080上皮细胞人纤维肉瘤[ MEM, 10% HI 胎牛血清和NEAA• 2010-11-16 11:33.回复2楼HT-29 上皮细 胞 人 结肠腺• 癌 McCoy 5A, 10% 胎牛血•蕊特生物 清• JEG-2 上皮细胞 人 绒毛膜癌 MEM, 10%胎牛血清MCF7 上皮细 胞 人 乳腺 癌 MEM, 10%胎牛血清 NEAA, 10ug/ml 腹岛素 KB 上皮细胞 人 口腔 癌 MEM, 10%胎牛血清和NEAA Saos-2 上皮细 胞 人 骨肉 瘤 McCoy5A, 15%胎牛血清 WT-38 上皮细 胞 人 胚胎肺 WISH 胞 膜 WS1 肤 NEAA HUVEC 胞 带BME, 10%胎牛血清 上皮细人BME,MEM,羊10%胎牛血清人 胚胎皮10%胎牛血清和内皮细人 脐F-12K, 10%胎牛血清和肝素盐100 Ug/ml EB-3 样 瘤 清 Raji 样 瘤 清 TM-9 胞 髓 清 Dau di 胞 液 清 H9 胞 瘤 清 HL-60 胞 血病 血清 Jurkat 胞 病 清 K-562 胞 病 清 U937淋巴人 Burkitt淋巴RPMI-1640, 10% 胎牛血淋巴人 Burkitt淋巴RPMI-1640, 10% 胎牛血成淋巳细人 骨髓瘤病人骨RPMI-1640, 10% 胎牛血成淋巴细人 淋巳瘤病人血RPMI-1640, 10% 胎牛血成淋巴细人 T细胞淋巴RPMT-1640, 20% 胎牛血成淋巴细人 早幼粒细胞白RPMI-1640, 20% 胎牛成淋巴细人 T细胞白血RPMI-1640, 10% 胎牛血成淋巳细人 骨髓性的白血RPMI-1640, 10% 胎牛血淋巴样 人 组织细胞淋巴瘤 RPMI-1640, 10% 胎牛血 清• 2010-11-16 11。