人胃癌血管特异性结合多肽的体内筛选及其三维结构模拟的初步分析
10页1、1人胃癌血管特异性结合多肽的体内筛选及其三维结构模拟的初步分析作者:郅敏,肖高铿,郅慧,董蕾,乔泰东,樊代明,吴开春 【关键词】 胃癌 【Abstract】 AIM: To identify and analyze a peptide binding specifically to blood vessels of human gastric cancer by phage displayed C7C peptide library in vivo and to model its three dimensional structure of the peptide by computer. METHODS: Animal models were established using subrenal capsular assay (SRCA) in immunosupressed mice implanted with human gastric cancer xenografts. The phage displayed C7C peptide library was injecte
2、d intravenously into mice. After 4 rounds of selection, 14 clones were picked up randomly and sequenced individually. The homing ability to human umbilical vein endothelial cells (HUVEC ) of peptide CGNSNPKSC was determined by cellELISA, with SGC7901, Eca109, LoVo and HepG2 cells as control. Its three dimensional structure of peptide CGNSNPKSC was also established by computer modeling techniques. RESULTS: Fourteen clones were obtained and they displayed two kinds 2of peptides. The binding abilit
3、y of a cyclic peptide of CGNSNPKSC toward HUVEC was higher than that of SGC7901, Eca109, HepG2 and LoVo cells. The three dimensional structure of the peptide of CGNSNPKSC was modeled successfully. CONCLUSION: The peptide of CGNSNPKSC has high binding ability to HUVEC and its stable structure and highly hydrophilic character help it form a flexible epitope of antigen. The peptide of CGNSNPKSC can be used in target therapy of tumor angiogenesis. 【Keywords】 gastric cancer; phage displayed peptide l
4、ibrary; angiogenesis; peptides; structure modeling 【摘要】 目的: 利用噬菌体随机环七肽库在小鼠肾包膜下荷人胃癌模型体内筛选能够与人胃癌血管内皮细胞结合的特异性分子,对其多肽进行计算机空间结构模拟分析. 方法:制备免疫抑制小鼠肾包膜下人胃癌移植瘤模型,用随机环七肽库在其体内进行 4 轮筛选,对随机获得的 14 个克隆进行测序. 通过细胞 ELISA 检测所得到的特异性小肽在脐静脉内皮细胞(HUVEC) ,SGC7901,LoVo,Eca109 及 HepG2 细胞系上的结合能力. 并通过计算机分子模拟多肽 CGNSNPKSC 的空间结构. 结果:成功获得14 个噬菌体克隆,并呈现出 2 种氨基酸序列,其中多肽CGNSNPKSC,相比于对照细胞 SGC7901, LoVo, Eca109, HepG2,其同 HUVEC 结合能力明显增强. 利用计算机技术成功地3模拟并分析了该多肽的空间结构. 结论:多肽 CGNSNPKSC 同血管内皮细胞结合能力较强;空间结构稳定,是一个高亲水多肽,更容易形成折曲的抗原表位,很有可能是一个极具肿瘤血管靶
《人胃癌血管特异性结合多肽的体内筛选及其三维结构模拟的初步分析》由会员油条分享,可在线阅读,更多相关《人胃癌血管特异性结合多肽的体内筛选及其三维结构模拟的初步分析》请在金锄头文库上搜索。
最新学校新冠肺炎疫情突发事件应急处置办法和流程
最新疫情防控期间学校用餐与食堂管理规定和师生一日流程图
最新Xx省xx区返学师生员工健康登记表
诊所简介
高中英语-第二单元-《the-United-Kingdom》课件-新必修5
新版新目标英语七年级下unit4--Don't-eat-in-class课件
新版PEP六年级英语unit6-how-do-you-feel-A-Let's-talk
安徽专版2018秋八年级英语上册Unit5Doyouwanttowatchagameshow第6课时习题课件新人教目标版
酸的和甜的教学课件.doc
Lesson-1-Where-do-we-go-from-here概要
Docker技术与实践
七年级英语下册-Unit-9-What-does-he-look-like(第3课时)(Grammar-Focus-3d)同步语法精讲精练课件-(新版
《I'm-going-to-do-the-high-jump》PPT课件3
高二英语外研版-必修5-Module-2-A-Job-Worth-Doing-Reading课件
英语片段教学-I-can-do-it
Adobe公司简介
安徽专版2018秋八年级英语上册Unit5Doyouwanttowatchagameshow第2课时习题课件新人教目标版
第二单元全课件Unit2-What-time-do-you-go-to-school-全课件
第18课-Windows的磁盘管理与安全
第12章--Windows-Server-2008路由及远程访问
2023-07-07 1页
2023-05-22 8页
2023-01-01 2页
2024-02-16 3页
2024-02-13 31页
2022-08-04 2页
2023-07-10 16页
2023-07-28 3页
2022-12-11 30页
2022-11-20 14页