葛根芩连汤论文：不同制备工艺葛根芩连汤抗感染作用的比较研究.docx

葛根苓连汤论文:不同制备工艺葛根苓连汤抗感染作用的比 较研究【中文摘要】通过对不同制备工艺的葛根苓连汤在体内、外抗感 染作用的对比研究,探讨传统方剂因制备工艺不同引起有效成分的含 量不同，随之出现的药效差异，为经方的二次开发提供思路方法：一、 三种不同工艺制备葛根苓连汤：MD、SD、EAI二、选用标准菌菌株球 菌和肠道杆菌共十种三、实验过程1、中药原液体外抑菌实验2、含 药血清抑菌实验3、体内抗感染实验结果：1、中药原液抑菌作用不 同,MD、SD、EAI三者比较,PV0.01,存在极显著差异；SD抑菌作用大 于MD,PV0.05,二者差异有统计学意义；EAI抑菌作用大于MD,PV 0.01，二者存在极显著差异2、含药血清抑菌作用不同,MD、SD、EAI 三者比较,PV0.01,存在极显著差异SD与MD抑菌作用差异无统计 学意义；EAI抑菌作用大于MD,PV0.05,二者差异有统计学意义3、 对小鼠耳廓急性炎症的缓解作用,MD、SD、EAI三者比较,PV0.01,存 在极显著差异SD作用大于MD,PV0.05,二者差异有统计学意义°EAI 作用大于MD,PV0.01，二者存在极显著差异结论：1、中药原液对 十种菌株均有抑制作用，以EAI作用最强。

2、空白对照血清无抑菌作 用，含药物血清对十种菌株抑制作用以EAI为最强3、三种制备工艺 葛根苓连汤对局部炎症缓解的作用EAI最强英文摘要】：The present study was aimed to investigate the comparative on anti-infection effects between different preparation technological of gegenqinlian decoction. We find the different preparation technological of traditional recipe can lead to the change of its active ingredients and the differences in its efficacy. We can find a new way to the secondary development of traditional recipeMethods:1. Preparate three different Gegenqinlian Decoction:MD, SD, EAI.2.Selected ten kinds of standard strains of bacteria and intestinal bacteria.3.the progress experiment3.1 Antibacterial medicine experiment3.2 Inhibition experiments with serum3.3 Anti-infection experiments in vivoResult:1.Different inhibitory effect of Chinese medicine liquid, MD, SD, EAI compare the three, P<0.01, significant differences exist; SD inhibition than MD, P<0.05, the difference was statistically significant; EAI large inhibition in MD, P<0.01, significant differences exist between the two 2.Inhibitory effect of serum containing different, MD, SD, EAI compare the three, P<0.01, significant differences exist. Inhibitory effect of SD and MD were no significant differences; EAI inhibition than MD, P<0.05, the difference was statistically significant.3. Acute inflammation of mouse ear Alleviation, MD, SD, EAI compare the three, P<0.01, significant differences exist. SD was larger than MD, P<0.05, the difference was statistically significant. EAI was larger thanMD, P<0.01, significant differences exist between the two.Conclusion:1. liquid on the ten kinds of Chinese strains are inhibited,2. No inhibitory effect of the control group. EAI strongest.3. three kinds of preparation Gegenqinlian Decoction on the role of local inflammation relief EAI strongest.【关键词】葛根苓连汤 制备工艺中药抑菌实验 血清药理学 抗 感染【英文关键词】Gegenqinlian Decoction preparation technological bacteriostasis experiment of the traditional Chinese medicine serum Pharmacology anti-infection【目录】不同制备工艺葛根苓连汤抗感染作用的比较研究 缩略语表 4-5 中文摘要 5-6 ABSTRACT 6-7 前言8- 9 文献综述 9-22 1葛根苓连汤9-15 1.1葛根苓连汤的来源和组成9 1.2葛根苓连制剂的制备方法9- 12 1.3葛根苓连汤的临床应用及疗效12-13 1.4葛根苓连汤的现代药理研究13-15 2中药抑菌作用研究15- 17 2.1单味中药抑菌作用研究15-16 2.2复方中药抑菌作用研究16 2.3中药抑制耐药菌株作用的研究16- 17 3血清药理学17-19 3.1血清药理学的提出及含义17 3.2含药血清的制备方法17-18 3.3葛根苓连汤血清药理学的研究进展18-19 4中草药改善局部炎症实验方法19-22 材料与方法22-28 1实验材料22-251.1实验菌种22 1.2实验中药22-231.3肉汤培养基 23 1.4实验动物 23 1.5主要试剂23-24 1.6主要仪器24-25 2实验方法25-282.1不同制备工艺葛根苓连汤主要成分含量测定25 2.2体外抑菌实验25-26 2.3血清药理学实验26-272.4改善局部炎症实验27 28 结果28-351不同制备工艺葛根苓连汤主要成分差异比较28-292二种制备工艺葛根苓连汤体外抑菌效果不同29-303含药血清抑菌效果不同30 32 4改善局部炎症效果不同32-35 讨论35-39 结论39-40 致谢40-41 参考文献41-50 个人简历50I【曲采买全文魁】1.399388.48 1_3 8 1_13.7 2.1 同时提供论文写作一对一和论文发表服务•保过包发一t说明】本文仅为中国学术文献总库合作提供七无涉版权。

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