pibv5-his昆虫表达载体说明.pdf

pIB/V5-His 编号编号 载体名称载体名称 北京华越洋生物 KCVECT147 pIB/V5-­‐His pIB/V5-­‐His 载体基本信息载体基本信息 载体名称载体名称: pIB/V5-His 质粒类型质粒类型: 昆虫表达载体 克隆方法克隆方法: 多克隆位点，限制性内切酶 启动子启动子: OplE2 载体大小载体大小: 3521 bp 5' 测序引物及序列测序引物及序列: OpIE2 Forward：CGCAACGATCTGGTAAACAC 3' 测序引物及序列测序引物及序列: 5'd[CGCAACGATCTGGTAAACAC]3' 载体标签载体标签: V5 Epitope Tag , His Tag (C-末端) 载体抗性载体抗性: 氨苄青霉素 真核筛选标记真核筛选标记: Blasticidin 克隆菌株克隆菌株: TOP10、DH5 宿主细胞（系）宿主细胞（系）: Sf9 、High Five 备注备注: -- 瞬表达瞬表达/稳表达稳表达: 瞬表达 组成型组成型/诱导型诱导型: 组成型 病毒病毒/非病毒非病毒: 非病毒 pIB/V5-­‐His 载体质粒图谱和多克隆位点信息载体质粒图谱和多克隆位点信息 pIB/V5-­‐His 载体简介载体简介 The InsectSelect BSD Kit features the pIB/V5-­‐His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells: • The OpIE2 promoter for constitutive expression • The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks • C-­‐terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-­‐V5 Antibody and rapid purification with nickel-­‐chelating resin pIB/V5-­‐His 载体序列载体序列 ORIGIN 1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG 61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT 121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC 181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC 241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC 301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT 361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA 421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC 481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG 541 CATCTGTTCG AATTTAAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA 601 TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGC GGTTCGAAGG 661 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA 721 TCACCATTGA GTTTATCTGA CTAAATCTTA GTTTGTATTG TCATGTTTTA ATACAATATG 781 TTATGTTTAA ATATGTTTTT AATAAATTTT ATAAAATAAT TTCAACTTTT ATTGTAACAA 841 CATTGTCCAT TTACACACTC CTTTCAAGCG CGTGGGATCG ATGCTCACTC AAAGGCGGTA 901 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 961 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 1021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 1081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 1141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 1201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 1261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 1321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 1381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 1441 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 1501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 1561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 1621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCGAAA CACGCACGGC 1681 GCGCGCACGC AGCTTAGCAC AAACGCGTCG TTGCACGCGC CCACCGCTAA CCGCAGGCCA 1741 ATCGGTCGGC CGGCCTCATA TCCGCTCACC AGCCGCGTCC TATCGGGCGC GGCTTCCGCG 1801 CCCATTTTGA ATAAATAAAC GATAACGCCG TTGGTGGCGT GAGGCATGTA AAAGGTTACA 1861 TCATTATCTT GTTCGCCATC CGGTTGGTAT AAATAGACGT TCATGTTGGT TTTTGTTTCA 1921 GTTGCAAGTT GGCTGCGGCG CGCGCAGCAC CTTTGCCGGG ATCTGCCGGG CTGCAGCACG 1981 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA 2041 CTAAACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC 2101 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG 2161 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGCGCAGA 2221 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC 2281 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTTCTTCT 2341 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT 2401 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGCAC TTCGTGGCCG 2461 AGGAGCAGGA CTGACACGTC CCGGGAGATC TGCATGTCTA CTAAACTCAC AAATTAGAGC 2521 TTCAATTTAA TTATATCAGT TATTACCCAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 2581 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 2641 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 2701 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 2761 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 2821 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 2881 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 2941 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 3001 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 3061 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 3121 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 3181 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 3241 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGT。