常用pGEX载体图谱.docx

Rosetta系列的体现菌株可以提供T7 RNA聚合酶，它能体现PET系列载体上的外源基因pGEX系列载体上的外源基因不需要T7 RNA聚合酶，一般的大肠杆菌经IPTG诱导即可体现Tac启动子是一组由Lac和trp启动子人工构建的杂合启动子，受Lac阻遏蛋白的负调节，它的启动能力比Lac和trp都强其中Tac 1是由Trp启动子的－35区加上一种合成的46 bp DNA片段(涉及Pribnow 盒)和Lac操纵基因构成，Tac 12是由Trp的启动子-35区和Lac启动子的-10区，加上Lac操纵子中的操纵基因部分和SD序列融合而成蛋白标签：A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.A monoclonal antibody against the myc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma BankpGEX4T1载体基本信息出品公司:GE别名:pGEX-4T-1, pGEX4T1, pGEX 4T 1质粒类型:大肠杆菌蛋白体现载体体现水平:高拷贝启动子:Tac克隆措施:多克隆位点，限制性内切酶载体大小:4969 bp5' 测序引物及序列:pGEX5':  GGGCTGGCAAGCCACGTTTGGTG3' 测序引物及序列:pGEX3': CCGGGAGCTGCATGTGTCAGAGG载体标签:N-GST载体抗性:Ampicillin 氨苄备注:复制子是pMB1产品目录号:27-4580-01稳定性:瞬时体现 Transient构成型:诱导体现病毒/非病毒:非病毒pGEX4T1载体质粒图谱和多克隆位点信息原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。

大肠杆菌的复制起点涉及OriC和OriHThrombin 凝血酶pGEX4T1载体简介pGEX4T1载体序列LOCUS pGEX-4T-1 4969 bp DNA circular SYNDEFINITION pGEX-4T-1ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            COMMENT     VNTAUTHORNAME||FEATURES Location/Qualifiers source 1..4969 /organism="pGEX-4T-1" /mol_type="other DNA" promoter 184..212 /label="tac_promoter" misc_feature 224..246 /label="M13_pUC_rev_primer" gene 258..977 /label="GST (variant)" /gene="GST (variant)" CDS 258..977 /label="ORF frame 3" misc_feature complement(1019..1041) /label="pGEX_3_primer" promoter 1307..1335 /label="AmpR_promoter" gene 1377..2237 /label="Ampicillin" /gene="Ampicillin" CDS 1377..2237 /label="ORF frame 3" rep_origin 2392..3011 /label="pBR322_origin" misc_feature 3309..4400 /label="lacI" CDS 3441..4400 /label="ORF frame 3" promoter 4449..4478 /label="lac_promoter" misc_feature 4492..4514 /label="M13_pUC_rev_primer" promoter 4513..4531 /label="M13_reverse_primer" CDS 4523..81 /label="ORF frame 2" misc_feature 4540..4695 /label="lacZ_a" promoter complement(4543..4559) /label="M13_forward20_primer" misc_feature complement(4552..4574) /label="M13_pUC_fwd_primer"pGEX4T2载体基本信息出品公司:GE别名:pGEX-4T-2, pGEX4T2, pGEX 4T 2质粒类型:大肠杆菌蛋白体现载体体现水平:高拷贝启动子:Tac克隆措施:多克隆位点，限制性内切酶载体大小:4970 bp5' 测序引物及序列:pGEX5':  GGGCTGGCAAGCCACGTTTGGTG3' 测序引物及序列:pGEX3': CCGGGAGCTGCATGTGTCAGAGG载体标签:N-GST载体抗性:Ampicillin 氨苄备注:复制子是pMB1产品目录号:27-4581-01稳定性:瞬时体现 Transient构成型:诱导体现病毒/非病毒:非病毒pGEX4T2载体质粒图谱和多克隆位点信息pGEX4T2载体简介pGEX4T2载体序列LOCUS pGEX-4T-2 4970 bp DNA circular SYNDEFINITION pGEX-4T-2ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            COMMENT     VNTAUTHORNAME||FEATURES Location/Qualifiers source 1..4970 /organism="pGEX-4T-2" /mol_type="other DNA" promoter 184..212 。