常见限制性内切酶识别序列
5页1、常见限制性内切酶识别序列(酶切位点)(BamHI 、EcoRI、HindIII 、Ndel、Xhol 等)Time : 2009-10-22 PM 15:38Author:bioerHits:7681 times在分子克隆实验中,限制性内切酶是必不可少的工具酶。无论是构建克隆载体还是表达载体,要根据载体选择合适的内切酶(当然,使用T载就不必考虑了)。先将引物设计好,然后添加酶切识别序列到引物5端。常用的内切酶比如BamHI、EcoRI、HindIII 、NdeI、XhoI等可能你都已经记住了它们的识别序列,不过 为了保险起见,还是得查证一下。下面是一些常用的II型内切酶的识别序列,仅供参考。先介绍一下什么是II型内切酶吧。The Type II restrictio n systems typically con tai n in dividual restrictio n en zymes and modificatio n en zymes en coded by separate gen es. The Type II restricti on en zymes typicall
2、y recog nize specific DNA seque nces and cleave at con sta nt positi ons at or close to that seque nee to produce 5-phosphates and 3-hydroxyls. Usually they require Mg 2+ ions as a cofactor, although some have more exotic requireme nts. The methyltra nsferases usually recog nize the same seque nee although some are more promiscuous. Three types of DNA methyltra nsferases have bee n found as part of Type II R-M systems formi ng either C5-methylcytos ine, N4-methylcytos ine or N6-methylade nine.酶类
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