NEB内切酶产品具体Buffer活性表.doc

NEBuffer Activity Chart for Restriction EnzymesNew England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100% activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffer activity reactions. In addition, the table also shows recommended reaction temperature, heat inactivation temperature and recommended diluent buffer. Please click here for additional information about: Unique NEBuffer Activity  | Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008.Click for the Chart Legend, Icon Description A | B | C | D | E | F | H | I | K | M | N | P | R | S | T | X | Z | EnzymeSupplied NEBuffer% Activity in NEBuffersHeatInactiva-tion °CIncuba.Temp.°CDiluent1234AatIINEBuffer 4050501006537AAcc65INEBuffer 3 + BSA1075100256537AAccINEBuffer 45050101008037AAciINEBuffer 32550100506537AAclINEBuffer 4 + BSA10100100No37BAcuINEBuffer 4 + SAM50100501006537BAfeINEBuffer 42550251006537AAflIINEBuffer 4 + BSA50100251006537AAflIIINEBuffer 3 + BSA2575100508037BAgeINEBuffer 11005010756537CAhdINEBuffer 4 + BSA257501006537AAleINEBuffer 41025101006537AAluINEBuffer 4100100751006537BAlwINEBuffer 450100101006537AAlwNINEBuffer 450100501006537AApaINEBuffer 4 + BSA255001006525AApaLINEBuffer 4 + BSA10010010100No37AApeKINEBuffer 3257510050No75BApoINEBuffer 3 + BSA1075100758050AAscINEBuffer 4010101006537AAseINEBuffer 3NR75100 ddNR6537BAsiSINEBuffer 4 + BSA501001001008037BAvaINEBuffer 41075101008037AAvaIINEBuffer 45075101006537AAvrIINEBuffer 4100100501008037BEnzyme    topSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentBaeGINEBuffer 11007510506537ABaeINEBuffer 4 + BSA + SAM50100501006525ABamHINEBuffer 3 + BSA75100100 dd100No37ABamHI-HF™NEBuffer 41005010100No37ABanINEBuffer 4 + BSA50100501006537ABanIINEBuffer 410010501008037ABbsINEBuffer 210010025756537BBbvCINEBuffer 450100101008037ABbvINEBuffer 210010025756537BBccINEBuffer 1 + BSA1005010506537ABceAINEBuffer 3 + BSA1001001001006537ABcgINEBuffer 3 + SAMNRNR100 ddNR6537ABciVINEBuffer 41005001006537CBclINEBuffer 350100100 dd75No50ABfaINEBuffer 47550101008037BBfuAINEBuffer 3075100106550BBfuCINEBuffer 4 + BSA10050251008037BBglINEBuffer 35075100506537BBglIINEBuffer 3107510010No37ABlpINEBuffer 45010010100No37ABmgBINEBuffer 3 + BSA025100106537CBmrINEBuffer 275100751006537BBmtINEBuffer 22510025506537BBpmINEBuffer 3 + BSA751001001006537BBpu10INEBuffer 31025100258037BBpuEINEBuffer 4 + SAM50100101006537BBsaAINEBuffer 4100100100100No37CBsaBINEBuffer 450100751008060BBsaHINEBuffer 4 + BSA501001001008037ABsaINEBuffer 475751001006550BBsaJINEBuffer 41001001001008060ABsaWINEBuffer 4 + BSA50100501008060ABsaXINEBuffer 47510010100No37BBseRINEBuffer 4100100751006537ABseYINEBuffer 31050100506537BBsgINEBuffer 4 + SAM5075501006537BBsiEINEBuffer 4 + BSA50100101008060ABsiHKAINEBuffer 4 + BSA50100100100No65BBsiWINEBuffer 3100100100258055ABslINEBuffer 31050100758055ABsmAINEBuffer 4501001001008055BBsmBINEBuffer 3751001001008055BBsmFINEBuffer 4 + BSA2550501008065ABsmINEBuffer 475100751008065ABsoBINEBuffer 21010010050No37ABsp1286INEBuffer 4 + BSA2525251006537ABspCNINEBuffer 4 + BSA + SAM10075101008025ABspDI。