常用载体通用引物.doc

载体名称上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMVCMV-30(825-854)CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH revpAcG2TpGEX-5not availablepACTT7EEVT3/EBVrevpACT2GAL4 ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site)PBRforBamPBRrevBampACYCDuet-l(MCSI)ACYCDuetUP1 PrimerDuetDOWN1pACYCDuet-1(MCSII)DuetUp2T7-TerminatorpAdEasy-1not availablenot availablepAdTrack-CMVnot availablenot availablepAS2-1GAL4 Bdfor/P24990pGAL4-BDrv/P31430pB42ADpB42ADFPB42ADRpBABEpBMN_5'pBacPAK8BAC1BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5'pBBR1MCST3/M13revT7/M13forpBD-GAL4pBD-GAL4-FPBD-GAL4-RpBI12135SNOSpBINDGAL4-BdforT3,EBVrevpBK-CMVM13F/T7M13R/T3pBluescriptllKS(+/-)T3/M13revT7/M13for (优先用 M13F)pBluescriptllSK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site)PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites)pBRforEcopBRrevHindpBR325(BamHI-site)PBRforBamPBRrevBampBR329(BamHI-site)PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3'BDpBridge(MCSII)not availablenot availablepBS-T IIT3/M13revT7/M13forpBS (AMP )T7T3pBSRT3T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1 a FwdBGHpBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrevpBV220pBV220FPBV220RpCAL-cT7/Tac-ProforT7-TerminatorpCAL-nT7/Tac-ProforT7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)pCANTB5ES1S6pCAT3-enhancerRVP3此载体无反向引物pCDFDuet-l(MCSI)ACYCDuetUP1 PrimerDuetDOWN1pCDFDuet-l(MCSII)DuetUP2T7TERpcDNA1.1CMV-Profor,T7pCDM8-3pcDNA2.1M13for/T7M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGH rev/SP6pcDNA3.1 (+/-)pcDNA3.1F/T7/CMV-ProforBGH revpcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGH revpcDNA3.1/myc-His(_A,_B,_C)T7/CMV-ProforBGH rev/c-mycrevpcDNA3.1/V5-His(_A,_B,_C)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Hygro (+,-)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Zeo(+.-)T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C)XpressforBGH revpcDNA4/HisMax-T0P0XpressforBGH revpcDNA4/HisMax-T0P0/lacZXpressforBGH revpcDNA4/myc-His(_A,_B,_C)T7,CMV-ProforBGH rev,c-mycrevpcDNA4/TOTREfor/CMV-ProforBGH revpcDNA4/V5-His(A,B,C)CMV-Profor,T7BGH rev,V5revpcDNA4-TET/0N/AMP+CMV-ProforpcDNA5/FRTT7/CMV-ProforBGH revpcDNA6/myc-His(A,B,C)CMV-Profor,T7BGH rev,c-mycrevpcDNA6/V5-His(A,B,C)CMV-Profor,T7BGH rev,V5revpCEP4pCEP-F/CMV-ProforEBVrevpCIT7 (17BASE)EBVrevpCI-neoT7 (17BASE)EBVrev,T3pCMS-EGFPT7T3pCMS-EGFPT7EBVrev,T3pCMV-3Tag-4AT3 /PFLAG-CMV-FT7pCMV5pCMV5FPCMV5RpCMV6CMV-ProforpCMV6-3、HGHrevpCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrevpCMV-HAPCMV-F,CMV-Profor,TREforPCMV-R , EBVrevpCMV-MYC(AMP+)PCMV-FPCMV-R, EBVrevpCMVmyc/nucCMV-ProforBGH rev,c-mycrevpcmv-scriptEXVectorT3T7pCMV-Sport6T7/M13forSP6/M13revpCOLADuet-1(MCSI)ACYCDuetUP1 PrimerDuetDOWN1pCOLADuet-l(MCSII)DuetUP2T7TERpCR2.1T7,M13forM13revpCR2.1-TOPOM13for,T7M13revpCR3.1T7BGH revpCR3.1-UniT7BGH revpCR4T3/M13revT7/M13forpCR4-BluntT3/M13revT7/M13forpCR4-TOPOM13rev,T3M13for,T7pCR-BluntM13revT7,M13forpCR-BluntIIM13rev/SP6T7/M13forpCR-Bluntll-TOPOM13rev/SP6M13for/T7pCRIIM13rev/SP6T7/M13forpCRII-TOPOM13rev/SP6M13for/T7pCR-ScriptSK(+)T7/M13forT3/M13revpCR-XL-TOPOM13revT7,M13forpCS2+(MCSI)SP6EBVrev,T7?pCS2+(MCSII)T3pSG5-3pDB_GAL4camT7PpDB_leuPDBLeu-5PDBLeu-3pDEST22DEST22FDEST22RpDEST15pGEX-5T7-TerminatorpDEST20pGEX-5pFastBac-3pDEST26TREfor/CMV-ProforT7/M13forpDisplayT7/CMV-Proforc-mycrevpDNR-LIB(MCS A)M13for,T7pSG5-3,M13RpDonarM13FM13RPDONR201PDONR-FPDONR-RpDONR207PDONR-FPDONR-RpDONR223M13FM13RpDriveT7/M13revSP6/M13forpDsRED1-C1(DsRed1-N-f)DsRed1-C-rpDsRed1-N1CMV-ProforRFP-Nrev,CMV-R?pDsRed2-N1(DsRed1-N-f)DsRed1-C-rpDsRed2-C1(DsRed1-N-f)DsRed1-C-rpDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3'pDsRed-ExpressdsRed1 N primer/CMV-FdsRed1 C primer/CMV-RpDSRED-monomer-N1(KAN+)dsRed1 N primerpDSRED-N1(KAN+)pEGFP-N-5'pDSRED-N-RpEASY-BluntM13FT7/ M13RpEASY-BluntSimpleM13FT7/ M13R （距离插入位点合适， 优先安排）PEASY-T1 SimpleM13FM13R （距离插入位点合适，优 先安排）pEASY-TIM13F/T7M13RpEASY-T3M13F/T7M13R/SP6pECFP-N(C)PECFP-N-5.PECFP-N-3,pEF/myc/cyto/ER/mito/nucpEF-5Pcdna3.1R/BGH revpEF1(4,6/myc-His)T7/pEF-5Pcdna3.1R/BGH revpEF1(4,6/V5-His)T7/pEF-5Pcdna3.1R/BGH revpEF1(4;6/His_A;_B;_C)T7/XpressforPcdna3.1R/BGH revpEF5/FRT/V5-D(-TOPO)T7/pEF-5Pcdna3.1R/BGH revpEGFPM1。