脑梗死载脂蛋白E基因多态性与气虚痰热证的关系_周正艳.pdf

7üµ«‘âEyÿŸÐ´££¥1"Ûž1, 2,Êi­1,ç½\1( 11海南医学院附属医院,海南海口 570102; 21贵阳中医学院,贵州贵阳 550002)K 1:目的:研究脑梗死载脂蛋白 E( ApoE)基因多态性与中医气虚痰热证候的关系b方法:运用聚合酶链反应 -限制性片段多态性( PCR- RFLP)技术检测 50例脑梗死气虚痰热证患者及 50例健康对照者的 ApoE基因多态性分布特征,并进行比较分析b结果:共发现 6种基因型,分别为 E2/2aE2/3aE2/4aE3/3aE3/4aE4/4b脑梗死气虚痰热证患者ApoE3/4基因型和 E4等位基因频率均高于健康对照组, E2等位基因频率低于健康对照组,两组比较差异具有统计学意义(P 0105),4UM-aŸYµs (íd9еs,FWM-aŸY µ V1Ÿ,nV1bV1 FŸYM-1 FYn3oÜ (M-(ß)7üµ´££F50 29 21 64. 9? 7. 2r BvF50 26 24 64. 6? 7. 5212 两组载脂蛋白E基因型分布的比较E2/27üµ´££FÃ_, E4/4rBvFÃ_, E3/3FÏ (¹Èny˜,sY]60%„70% ,y˜Ôq1 µsíA÷Ÿ(P = 0116> 0105);FE3/4y˜Ôq1 µsµA÷Ÿ(P = 01012 0105); E4©ÊyÔq7üµF( 21% )üAÚ¿r BvF( 4% ) (P= 01004¯,3 A[12]b5…E#Ï„6ª:/Ar3,3£,£3„90b/$; VßäÎ7bL,9 V•<¹ª,Û@›,í)‚ž0b‚¥3‚JyÍaf½yÍ,F­©Ìây͝ÁZ2´£˜Ï„?hq Ú,«‘âEyÿŸ¥_©¿7üµ´££µ*ùaç]il,¹ÏDO£‚Í„D*ù]Í4G bÏD£©^”$M Ÿ"5Ll¥9²,µ"~¶¥=d,VC"Ÿ,^“z¥"5V˜"Ÿ¥ùî`bÛ"¦ ËyF9Ã¥'|›î„/ªyF0H}¥ž Ÿ, æ¨~¶¥DNAÿŸ'Æ)Ð%h#207# 辽宁中医杂志 2010年第 37卷第 2期 ¥ÏD£©"Ÿ­W¥1",µ V?¹yÿŸL@ñ8Ä"5ÍÏ?è×1T¨,g V¹³dÏD]hsÍash]Í4s0£Ü¥£ b+çyÿŸg^£©™îÐ8É Ë˜5?®­Wi1 ó¥s0$[13]b3€¥ùîÏD²†]͹u7üµ µBç¥il,µ€¿ÉB„'ÆÐ ”Zb• IÓD[ 1] $ ,¦b…,ñI:,©.3h„7üÖÑ€«‘âByÿŸùî[ J].Ï¿Ó5h½, 1999, 27( 1): 13- 16.[ 2] De Geest B, Zhao Z, Collen D, et a.l Effects of advenovirus mediatedhuman apoA-I ge-ne transfer on neointima formation after endothelialdenudation in apoE deficientm ice [ J]. Circulation, 1997, 96: 4349-4356.[ 3] Ï¿*Ü SÐö.ò Ë7Ó5%h1Ä[ J].Ï¿*Ü S½, 1996, 29( 6): 379- 380.là°ù: 2009- 05- 16T€eº:u’ó( 1980- ),3,„~lé¦,D=,«V,VYÏD0ç]Ï„ÐÓ5Ÿúw¥ùîb[ 4] ¢ƒ‡.Ï00"5ùð5(k›) [M ].Ø:ÏSD0 S/ñ, 2002: 99- 104.[ 5] ¦ß¿,aIA,S£,©.7üµÑ€«‘âEyÿŸ+Äùî[ J].ÏS8†"5, 2006, 22( 2): 118- 120.[ 6] Û ,,¿¶ù, Ùk),©.«‘âEyÿŸÐ7üµ¥1"[ J].ÏS7Ó5h½, 2006, 3( 6): 263- 266.[ 7] Paik YK, Chang DJ, Reardon CA, et a.l Nucleotide sequence andstructure of the human apolipoprotein E gene[ J]. ProcN atlA cad SciUSA, 1985, 82: 3445- 3449.[ 8] 1, Ù£ Á,¦? ë,©.«‘âEÿŸÐ7üµ#« Ë}†1"¥ùî[ J].ÏSh Ø3 ؽ, 2002, 18( 7): 826- 829.[ 9] ChowdhuryA H, Yokoyama T, Kokubo Y, et a,l A polipoprotein E ge-netic polymorphism and stroke subtypes in a Bangladeshi hospita-lbased study[ J]. J Epidem io,l 2001, 11: 131- 138.[ 10] çÏ³,o½­,æö,©.«‘âEyÿŸÐ ºM3h„7üµ¥1"[ J].Ï¿ ºM7Ó5h½, 2005, 7: 139.[ 11] + á¿, O 7¶, ÙTq,©.«‘âEyÿŸÐ7üµ¥M1Ÿs[ J].Ï„Ð*Ü%h½, 2003, 20( 10): 445- 447.[ 12] x .ÏDÏ„Ъ[ J].C} B¯, 2000, 4( 5): 784.[ 13] Ãt2,ç.‚«‘âE#yÿŸÐÏD|£©¥1"[ J].ÏSÏD$Dн, 2002, 8( 12): 13- 15.$ŸŒÓŸÏ„ªª©ÿ?h|¥£©1Í+˜u’ó1,†–{1,” ü1,ÔN–2( 11北京中医药大学东直门医院,北京 100700; 21北京中医药大学东方医院,北京 100078)K 1:目的:研究缺血性中风后认知功能减退证候要素及演变的特征b方法:对新发脑梗死非痴呆入院患者筛选后应用蒙特利尔认知评估量表 (北京版)动态多时点评定认知功能把握特征后分组,并进行组间证候要素比较b结果:组间比较火a痰a气虚a阴虚a肾虚出现显著差异b中风急性期火证突出,恢复期痰证a气虚证a阴虚证明显,肾虚贯穿始终b结论:火a痰a气虚a阴虚a肾虚很可能为中风后认知功能减退的主要证候要素b1oM:缺血性中风;认知功能;证候要素Ïms Ë|: R743. 3 ÓDSM’: A ÓcI|: 1000- 1719( 2010) 02- 0208- 03Characteristic of TCM Syndrome Factors of Cognitive Function Impairm ent After Ischem ic StrokeZHAO Zhen-wu1, XIE Ying-zhen1, MA H ong-m ing1, MENG Fan-xing2( 11Dongzhim enH osp ital, Beijing University of ChineseM edicine, Beijing 100700, China;21DongfangH ospital, Beijing University of ChineseM edicine, Beijing 100078, China)Abstract: Objective: To study characteristic of syndrom e factors and their evolvem entof cognitive function im pairm ent after is-chem ic stroke. M ethods: The cognitive function of patients in hospitalw ith cerebral infarction no dem entia were evaluated dynam -icallyw ithM ontrealCognitive Assessment(MoCA ) Beijing Version after IQCODE screens atdifferentphase points. Its characteris-tic was abstracted and used to grouping. Each syndrom e factorwas com pared within groups. Results: The rem arkable factors aftercomparison ofgroups are huo, tan, qideficientcy, yin deficiency and kidney deficiency. H uo is prom inent at acute period and tan,qideficiency and yin deficiency are distinct later. K idney deficiency runs through all the tim e. Conclusion: The research indicatesthat them ain factors inducing cognitive function im pairm ent of ischem ic stroke in all probability are huo, tan, qi deficienc。