罗氏电化学发光免疫分析仪项目推荐稀释比例.doc

Claudia Schäfer, Global Customer Support, Penzberg  4486 MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys® 2010, cobas e 411 Elecsys® Dilution Recommendations Autodilution possible for bold mentioned dilution ratios  Dilution not necessary due to the broad measuring range or not possible e.g. can not be diluted because of changing in the concentration of the binding proteins alters this equilibrium.@ The autoantibodies are heterogeneous and this gives rise to non-linear dilution phenomena.* Please check the package insert.STAT for Elecsys 2010, cobas e 411, cobas e 601 (SW version 04-03 onwards) and cobas e 602# Diluent Universal can be used to dilute the samples. Auto-dilution is not possible as assay uses a three-step method.  Use Elecsys® Diluent Universal for automatic sample predilution. Autodilution not possible.Diluent ratio Version 4.6May 20111/5Reagent typeTestRecommended dilution ratiosConcentration of diluted sample must be FT3 FT4 T3 T4 TSH1:10 with Diluent MA> 10 µIU/ml T-uptake TG1:5 with Diluent Universal> 50 ng/ml Anti-TG@ Anti-TPO@ 1:5 with Diluent Universal manually> 100 IU/mlThyroid Function TestAnti-TSHR1:5 or 1:10 with Anti-TSHR negative human sera*> 4 IU/lCK-MB (STAT)1:2 with Diluent MA> 50 ng/ml Digoxin1:2 with Diluent Universal> 2.5 ng/ml>3.2 nmol/l Digitoxin1:2 with Diluent Universal> 30 ng/ml> 39 nmol/l Myoglobin (STAT)1:10 with Diluent Universal> 50 ng/ml proBNP II (STAT only cobas e 601/2)1:2 with Diluent Universal> 15000 pg/ml > 1770 pmol/l Troponin I (STAT)1:10 with Diluent MA> 3 µg/l > 3 ng/ml Troponin T (STAT)1:10 with Diluent Universal> 1 µg/l > 1 ng/mlCardiacTroponin T hs (STAT )1:10 with Diluent MA> 1000 ng/l ACTH Cortisol1:10 with Diluent Universal * (for urine samples)> 50 nmol/l > 1.8 µg/dl DHEA-S1:5 with low concentrated human sera*> 1.5 µmol/l > 45 µg/dl Estradiol II1:5 with Diluent MA> 1835 pmol/l > 500 pg/ml FSH HCG+  HCG (STAT)1:100 with Diluent Universal*> 100 mIU/mlHormonesLHClaudia Schäfer, Global Customer Support, Penzberg  4486 MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys® 2010, cobas e 411 Elecsys® Dilution RecommendationsDiluent ratio Version 4.7August 20112/5Reagent typeTestRecommended dilution ratiosConcentration of diluted sample must be Progesterone II1:10 with Diluent Estradiol / Progesterone  *> 6 nmol/l > 2 ng/ml Prolactin II1:10 with Diluent Universal> 50 µIU/ml, > 2.4 ng/ml SHBG1:10 with Diluent MA> 20 nmol/l Testosterone II C-Peptide (serum and urine)1:10 with Diluent MA> 4 ng/ml > 1.3 nmol/lDiabetesInsulin PlGFPreeclampsia sFLT-1* Free ßhCG1:10 with Diluent Universal> 19 IU/lDown`s syndrome screeningPAPP-A1:10 with Diluent Universal> 500 mIU/lAFP1:50 with Diluent Universal> 20 U/ml, > 24 ng/ml CA 125 II1:5 with Diluent Universal> 1000 U/ml CA 15-3 II  1:10 with Diluent Universal (only 1:10 possible on E170 / cobas e 601/2)> 30 U/mlCA 19-91:10 with Diluent Universal> 50 U/ml CA 72-41:2 with Diluent Universal> 150 U/ml CEA1:50 with Diluent Universal> 20 ng/ml Cyfra 21-11:2 with Diluent Universal> 250 ng/ml HE41:20 with Diluent MA> 75 pmol/l NSE1:2 with diluent NSE > 50 ng/ml Free PSA Total PSA1:50 with Diluent Universal> 2 ng/mlTumor MarkerS 1001:5 with S 100 analyt free human serum or CalSet 1> 1 µg/lFree ßhCG1:10 with Diluent Universal> 19 IU/lDown`s syndrome screeningPAPP-A1:10 with Diluent Universal> 500 mIU/lVitamin B121:2 with Diluent Universal> 1000 pg/ml, >738 pmol/l Ferritin1:50 with Diluent Universal> 40 ng/ml Folate III1:2 with Diluent Universal> 10 ng/ml, > 22.7 nmol/lAnaemiaRBC Folate1:2 with 0.2% ascorbic acid solution>16.2 nmol/l > 8 ng/ml ß-CrossLaps hGH1:2 with Diluent Universal> 25 ng/ml N-MID Osteocalcin1:5 with Diluent Universal> 60 ng/ml Total P1NP1:2 with Diluent Universal> 100 µg/l PTH (STAT) PTH (1-84)1:2 with Diluent Universal> 1150 pg/nl > 122 pmol/lBoneVitamin D total1:2 with Diluent Universal> 35 ng/ml > 87.5 nmol/l VariaIgE II1:20 with Diluent Universal> 125 IU/ml, > 300 ng/ml Rheumatoid Anti-CCP1:2 to 1:5 with neg. human Claudia Schäfer, Global Customer Support, Penzberg  4486 MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys® 2010, cobas e 411 Elecsys® Dilution RecommendationsDiluent ratio Version 4.7August 20113/5Reagent typeTestRecommended dilution ratiosConcentration of diluted sample must be Arthritisserum pool* Brahms PCT1:4 with neg. human sera> 1.0 ng/mlSepsis IL 61:10 with Diluent MA> 50 pg/ml Anti-HAVwith Diluent Hepatitis A > 20 IU/l Anti-HAV IgM # Anti-HBc#* Anti-HBc IgM # Anti-HBe#* HBeAg#* Anti-HBs1:100 with Diluent Universal> 10 IU/ml HBsAg / HBsAg II* HBsAg II quant1:100 or 1:400 depending on the used system with Diluent HepB*Infectious DiseasesAnti-HCV* RetrovirusesHIV Ag* HIV combi* HIV combi PT* CMV IgG1:20 with Diluent Universal  15 U/ml CMV IgG Avidity1:20 with Diluent Universal* CMV IgM* HSV-1 IgG* HSV-2 IgG* Rubella IgG1:20 with Diluent Universal> 10 IU/l Rubella IgM* Toxo IgG1:20 with Diluent Universal>。