刺络放血治疗膝关节骨性关节炎模型兔的实验研究兔的实验研究.pdf

摘 要 目的：观察刺络放血治疗膝关节骨性关节炎（ osteoarthritis of knee， KOA）模型兔，通过实验研究解释刺络放血治疗 KOA 的可能作用机理 ， 为临床刺络放血治疗 KOA进一步提供科学依据 方法：将 32 只新西兰大白兔 ， 随机分为 4 组，即空白组（ A 组）、模型组（ B组）、刺络放血组（ C组）和药物治疗组（ D组） ，每组 8只将 A组的兔子正常饲养，不作任何处理；对 B 组的兔子只造模；对 C 组的兔子造模成功后进行刺络放血治疗，每周两次；对 D 组的兔子造模成功后每天给予药物治疗，共治疗 8 周治疗结束后，检测其骨内压数值、血清中 IL-lβ 、 TNF-α的 含量 结果： .B组与 A组比较， B组骨内压高于 A组，统计有显著性差异（ p＜ 0.01），说明造模成功； C 组及 D 组与 B 组比较，骨内压明显降低，统计有显著性差异（右侧 p＜ 0.01)， C 组与 D 组比较差异无统计学意义（ p>0.05），说明刺络放血疗法和该药物均可有效降低骨内压，其治疗效果相 当 .B组与 A组相比，血清中 IL-lβ、TNF-α含量 显著升高（ p＜ 0.01）； C组和 D组与 B组比较，血清中 IL-1β含量 显著降低，统计有显著性差异（ p＜ 0.01），血清中 TNF-α含量明显降低，统计有差异（ p＜ 0.05）， C组与 D组比较差异无统计学意义（ p>0.05），说明刺络放血疗法和该药物均可降低血清中细胞因子 IL-1β、 TNF-α含量。

结论： 刺络放血疗法能有效降低骨内压，进一步研究证明刺络放血疗法能降低血清中 IL-lβ、 TNF-α含量这些可能是刺络放血疗法治疗 KOA的作用机制之一 关键词 ： 刺 络放血疗法； KOA；骨内压； IL-lβ； TNF-α； Abstract Experimental Study of Osteoarthritis Rabbits Models Treated by Bloodletting Therapy Objective: To observe the osteoarthritis of knee (KOA） rabbit modles treated by blood-letting puncture therapy and explain the possible mechanism in treating KOA through experimental study, providing scientific evidence that can be used to treat KOA clinically. Methods: 32 New Zealand rabbits were randomly divided into four groups: the blank group(group A),the model group(group B),the blood-letting group(group C) and the drug group(group D),eight rabbits in each group. In group A,the rabbits were fed normally without doing other managements.In group B, rabbits were built into models.In group C,the rabbits were treated by blood-letting therapy after having been moulded successfully, twice per week.In group D,after having been moulded successfully, the rabbits were given medication every day,the total treatment lasting for eight weeks. Then, detect the intraosseous pressure value and the volume of IL-lβ and TNF-α in the serum. Results: Compared with group A, the intraosseous pressure of the rabbits in group B is higher than that in group A. The statistic shows that there exists significant difference（ p＜0.01） , which means that the models were built successfully. Compared with group B,the intraosseous pressure of the rabbits in group C and D decreases significantly and significant difference was observed （ right side p＜ 0.01). The difference between group C and D has no statictic meaning（ p>0.05） ,which means both blood-letting therapy and drug can decrease intraosseous pressure, with almost the same effect. Compared with group A, the volume of IL-lβ and TNF-αin the serum in group B significantly increase (p 0.05） , which means both blood-letting therapy and drug can decrease the volume of IL-1β、 TNF-α in the serum. Conclusion: The blood-letting puncture therapy can effectively reduce the volume of intraosseous pressure, and the volume of IL-lβ and TNF-α in the serum, which may be one of the mechanism for blood-letting puncture therapy to treat knee osteoarthritis. Key words: blood-letting puncture therapy; KOA; intraosseous pressure; IL-lβ; TNF-α; Arthor： Dongsheng Liang Tutor ： Shaoming Liu 目 录 引 言 ............................................................................................................................... 1 正 文 ............................................................................................................................... 3 一 文献研究 ..................................................................................................................... 3 （一） 中医学对膝关节骨性关节炎的认识 ............................................................ 3 （二） 现代医学对膝关节骨性关节炎的认识 ......................................................... 5 （三） 膝关节骨性关节炎的临床治疗状 况 ............................................................ 8 3.1 现代医学对膝关节骨性关节炎的治疗状况 ................................................. 8 3.2 中医学对膝关节骨性关节炎的治疗状况 ..................................................... 9 二 实验研究 ................................................................................................................. 13 （一）实验材料 ....................................................................................................... 13 （二）实验方法 ....................................................................................................... 14 三 实验结果 ................................................................................................................... 18 四 讨论 ........................................................................................................................... 20 五 结论 ........................................................................................................................... 24 六 存在的问题与展望 .................................................................................................... 25 结 语 ............................................................................................................................. 26 参考文献 ......................................................................................................................... 27 附 录。