痛风宁颗粒对鸡痛风模型血清黄嘌呤氧化酶活性和肾功能的影响.doc

痛风宁颗粒对鸡痛风模型血清黄嘌呤氧化酶活性和肾功能的影响专 业：中医骨伤科学研究生: 赖 震导 师: 周金水 主任医师、教授中　文　摘　要背景：痛风性关节炎是由于长期嘌呤代谢紊乱、血尿酸增高导致尿酸结晶沉积在关节及其周围组织而致组织损伤的一种疾病随着我国人民生活水平的提高，饮食结构的改变，痛风性关节炎的发病率有明显上升的趋势，对人民的健康已构成潜在的威胁西医方面目前尚缺乏治疗痛风性关节炎的理想药物，传统中医药治疗痛风性关节炎具有许多优势，临床和实验研究均显示有较好的疗效．且无明显毒副反响因此研究预防和治疗痛风性关节炎的中药制剂意义重大痛风宁方〞课题组前期研究发现痛风宁颗粒具有较好降血尿酸及抗炎、消肿、活血、镇痛作用，临床疗效好且无明显毒副作用本课题是在前期研究根底上对痛风宁颗粒疗效机理的探讨目的：通过观察痛风宁颗粒对鸡痛风模型血清黄嘌呤氧化酶活性和肾功能的影响，进一步探讨痛风宁颗粒防治痛风性关节炎的作用机理，为该药的开发应用打下根底方法：采用90日龄“海兰〔Hyline〕蛋鸡〞70只，在动物房适应性喂养5天后，按随机原那么抓取8只作为空白组，予以正常蛋鸡饲料喂养；另随机抓取8只作为痛风宁颗粒预防组，给予痛风造模饲料和痛风宁颗粒喂养。

其余实验鸡均按课题组前期制定的造模方法，给予痛风造模饲料喂养，以诱发痛风性关节炎模型形成后，模型鸡再随机分为5组，分别为痛风宁颗粒治疗组、别嘌呤醇治疗组、苯溴马隆治疗组、秋水仙碱治疗组和模型组，每组8只治疗观察4组在给予正常蛋鸡饲料喂养外，各组还分别给予相应药物〔痛风宁颗粒、别嘌呤醇、苯溴马隆、秋水仙碱〕喂养，模型组那么仅给予正常蛋鸡饲料喂养在实验前、实验7天、14天、21天测量各组实验鸡跗踝关节周径；在实验7天、14天、21天检测各组实验鸡血尿酸含量；在实验21天检测各组实验鸡血清黄嘌呤氧化酶活性、血尿素氮、血肌酐含量及关节腔滑液白细胞数 结果：　1. 实验14天后，痛风宁颗粒预防组血尿酸、跗踝关节周径与正常组相比无明显变化〔P>0.05〕；实验21天后，痛风宁颗粒预防组与正常组相比，血尿素氮、血肌酐含量无明显变化〔P>0.05〕；2. 实验21天后，痛风宁颗粒预防组与正常组相比，血XOD值没有显著升高〔P>0.05〕；痛风宁颗粒治疗组、别嘌呤醇治疗组与模型组相比，血XOD值有显著降低〔P<0.01〕； 3. 实验21天后，痛风宁颗粒治疗组、别嘌呤醇治疗组、苯溴马隆治疗组与模型组相比，血尿酸、血尿素氮、血肌酐含量显著降低〔P<0.01〕；秋水仙碱治疗组与模型组比拟血尿酸、血尿素氮、血肌酐含量没有显著差异〔P>0.05〕；4. 实验21天后，痛风宁颗粒治疗组、秋水仙碱治疗组与模型组相比，跗踝关节周径，关节液白细胞计数显著减少〔P<0.01〕。

而苯溴马隆治疗组、别嘌呤醇治疗组与模型组相比关节周径、关节液白细胞计数没有显著差异〔P>0.05〕结论：　1. 痛风宁颗粒可以降低痛风模型鸡血清黄嘌呤氧化酶活性；2. 痛风宁颗粒可以降低痛风模型鸡血尿素氮、肌酐含量和血尿酸含量，可以有效预防痛风性肾病的发生；3. 痛风宁颗粒可以有效预防和减轻鸡痛风性关节炎症；4. 痛风宁颗粒治疗鸡痛风性关节时具有良好的抗炎消肿的作用主题词　关节炎，痛风性/中医药疗法 尿酸/血液 黄嘌呤氧化酶/血液 血尿素氮 肌酸酐 鸡 动物 @痛风宁颗粒课题来源：本课题受“福建省科技厅科技攻关〔三项〕重点课题：中药三类新药痛风宁颗粒剂的研制开发，批号：2003Y023；福建省教育厅课题：痛风宁颗粒的质量标准及剂型稳定性研究，批号：JA03119”工程经费资助The Effect of Tongfengning granule on Xanthine Oxidase、Blood Urea Nitrogen、Creatinine in gouty arthritis hensMajor：Orthopedics and Traumatology of TCMGraduate: lai zhenTutor：Prof. zhou jinshuiAbstractBackground：Gouty arthritis is a kind of disease which is arise by the long-term purinemetabolism disorder, the blood uric acid advances causes the uric acidcrystal deposition in the joint and hypodermic. Along with the level of the people’s lives enhancement, Diet structure change, The gouty arthritis disease incidence rate has the obvious rise in our country, Posed the latent threat to people's health. At present clinical still lacked the ideal medicine of gouty arthritis, Clinical and the experimental studies have demonstrated that the chinese medicine is effective, and has no obvious poisonous and side reaction, Therefore the research of traditional Chinese medicine to prevention and treatment gouty arthritis is worthy. The prophase study of our team shows that the Tongfengning granule had good effect on anti-inflammatory, antioncotic, and analgesic action and the good effect of promoting blood circulation. It is significant to develop the patent medicine that is effective on treating gouty arthritis. On the base of prophase study of our team, the further study is to investigate the effect of Tongfengning granule On XOD、BUN、CR in gouty arthritis hens, and Build the foundation for this medicine clinical application.Objective： To investigate the effect of Tongfengning granule On XOD、BUN、CR in gouty arthritis hens,and discus the mechanism of Tongfengning granule in preventing and treating gouty arthritis. Methods： After 5 days’ adaptable feed,90-day old Hyline hens were fed by forage containing high nucleoprotein ,high calcium and limiting water ingestion to induce gouty arthritis. Allopurinol，benzbromarone，colchicine were used as control. XOD,Blood UA,BUN, Cr, swelling of joint and the quantity of leucocyte in joint fluid were observed.Results： 1. 14 days later, the hens of Tongfengning granule prevent group, compared with normal group’s, had no significant difference in serum UA and weekly of gambrel join (P>0.05),21 days later, the hens of Tongfengning granule prevent group, compared with normal group’s, had no significant difference in serum BUN and Cr( P>0.05);2. 21 days later, the hens of Tongfengning granule prevent group, compared with normal group’s, had no significant difference in XOD (P>0.05), in hens of Tongfengning granule treat group and Allopurinol treat group, compared with that of model group, had significant difference in the level of serum XOD (P<0.01).3. 21 days later ,in hens of Tongfengning granule treat group、Benzbromarone treat group and Allopurinol treat group, compared with that of model group, the level of serum UA,BUN, Cr declined obviously (P<0.01),but in hens of Colchicine treat group, compared with that of model group, had no significant difference in UA,BUN, Cr (P>0.05);4. 21 days later ,in hens of Tongfengning granule treat group、Colchicine treat group, compared with that of model group, the swelling of gambrel reduced obviously and the quantity of leucocyte in joint fluid also declined obviously (P<0.01),but in hens of Benzbromarone treat group and Allopurinol treat group, co。