《同尾酶汇总表》word版.doc

连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的酶切位点以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性如果有同裂酶存在，则只列出其中的一个酶本表中列出的内切酶均为已商品化的内切酶识别序列不够严谨的内切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列"-"表示连接产物不能被再切割酶连接到可进行切割的酶Acc65 I (G/GTACC)Ban I (G/GTACC)Acc65 I, Ban I, Kpn I, Nla IV, Rsa I BsiW I, BsrG IRsa IAcc I(GT/CGAC)Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I--(GT/CGAC)Cla I, BstB I, Taq ITaq IAci I(C/CGC) Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I--BsaH I (GR/CGCC), HinP1 I, Nar IAci IHpa IIHpa IIAcl I (AA/CGTT)Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq I--Afl III(A/CGCGT)Asc I, BssH IIBstU I, Hha I(A/CATGT)BspH I, Nco I, Pci INla III(A/CGCGT)Mlu IAfl III, BstU I, Mlu IAge I (A/CCGGT)Ava I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW I, BspE IBsaW I, Hpa IIBsrF I (A/CCGGT), SgrA I (CA/CCGGTG)Age I, BsaW I, BsrF I, Hpa IINgoM IVBsrF I, Hpa IIApa I (GGGCC/C)Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 IApaL I (G/TGCAC)Sfc I (C/TGCAG)Bsg IApo I(A/AATTY)EcoR IApo I, Tsp509 I(G/AATTY)EcoR IApo I, EcoR I, Tsp509 I(R/AATTY)Mfe I, Tsp509 ITsp509 IAsc I (GG/CGCGCC)Afl III (A/CGCGT), Mlu IBstU I, Hha IBssH IIBssH II, BstU I, Cac8 I, Hha IAse I (AT/TAAT)Bfa I, Csp6 I, Nde I--Mse IMse IAsiS I(GCGAT/CGC) BsiE I (CGAT/CG)Dpn II, Pvu IPac IMse IPvu IDpn II, Pvu IAva I(C/CCGGG)Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)Hpa II, Nci I, ScrF I(C/TCGAG)Xho IAva I, Taq I, Xho I(C/TCGAG)Sal ITaq I(C/CCGGG)Xma IAva I, BsaJ I, Hpa II, Nci I, ScrF I, Sma IAva II (G/GWCC)PpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IRsr IIAva II, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 IAvr II (C/CTAGG)Nhe I, Spe I, Xba IBfa ISty I (C/CTAGG)Avr II, Bfa I, BsaJ I, Sty IBamH I (G/GATCC)Bcl I, Dpn IIAlw I, Dpn IIBgl II, BstY I (R/GATCY)Alw I, BstY I, Dpn IIBstY I (G/GATCC)Alw I, BamH I, BstY I, Dpn II, Nla IVBan I(G/GTACC)Acc65 IAcc65 I, Ban I, Kpn I, Nla IV, Rsa I(G/GCGCC)Kas IBan I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV(G/GTACC)BsiW I, BsrG IRsa IBan II(GGGCC/C)Apa I, Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GAGCT/C)Bsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac IBcl I(T/GATCA) BamH I, BstY I (R/GATCY)Alw I, Dpn IIBgl II, Mbo IIDpn IIBfa I (C/TAG)Ase I, Csp6 I, Mse I, Nde I--Bgl II (A/GATCT)BamH I, BstY I (R/GATCY)Alw I, BstY I, Dpn IIBcl I, Dpn IIDpn IIBsaH I(GR/CGYC)Acc I (GT/CGAC), Cla I, BstB I, Taq I--(GA/CGYC)Aci I, HinP1 IHga I(GG/CGYC)Aci I, HinP1 IHha I(GG/CGYC)Hpa IIAci I(GA/CGYC)Nar IBsaH I, Hga I(GG/CGYC)Nar IBan I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaW I (W/CCGGW)Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)Age I, BsaW I, BsrF I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBspE IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVBsrF I, Hpa IINgoM IVHpa IIBsiE I(CGAT/CG)Pac IMse I(CGAT/CG)Pvu IBsiE I, Dpn II, Pvu I(CGGC/CG)Sac IIAci IBsiHKA I(GTGCA/C) Bsp1286 I (GTGCA/C)BsiHKA I, Bsp1286 IBsp1286 I (GAGCA/C)BsiHKA I, Bsp1286 IBsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac INsi I--Pst I, Sbf IBsg IBsiW I (C/GTACG)Acc65 I, Ban I (G/GTACC), BsrG IRsa IBsp1286 I(GGGCC/C)Apa I, Ban II (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GTGCA/C)BsiHKA IApaL I, BsiHKA I, Bsp1286 I(GGGCC/C)Ban II (GGGCC/C)Ban II, Bsp1286 I(GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac I(GWGCW/C)BsiHKA IBsiHKA I, Bsp1286 I(GTGCA/C)Nsi I--(GTGCA/C)Pst I, Sbf IBsg IBspE I (T/CCGGA)Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)BsaW I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVHpa IIBspH I (T/CATGA)Afl III (A/CATGT), Nco I, Sty I (C/CATGG)Nla IIIBsrF I(A/CCGGY)Age I, BsaW IAge I, BsaW I, BsrF I, Hpa II(G/CCGGY)Age I, BsaW I, NgoM IVBsrF I, Hpa II(R/CCGGY)Ava I (C/CCGGG), Xma IHpa II, Nci I, ScrF I(A/CCGGY)BsaW I, BspE IBsaW I, Hpa II(R/CCGGY)BsaW I, BspE IHpa II(G/CCGGY)NgoM IVBsrF I, Cac8 I, Hpa II, Nae I(CR/CCGGYG)SgrA IBsrF I, Hpa IIBsrG I (T/GTACA)Acc65 I, Ban I (G/GTACC), BsiW IRsa IBssH II (G/CGCGC)Afl III (A/CGCGT), Mlu IBstU I, Hha IAsc IBssH II, BstU I, Cac8 I, Hha IBstB I (TT/CGAA)Acc I (GT/CGAC), Cla I, Taq ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I--BstY I(A/GATCY)BamH I, Bgl IIAlw I, BstY I, Dpn II(G/GATCY)BamH IAlw I, BamH I, BstY I, Dpn II, Nla IV(R/GA。