免疫组化评分标准- 精选新修订.pdf

免疫组化评分标准：免疫组化评分标准： The immunohistochemical staining results were assigned a mean score considering both the intensity of staining and the proportion of tumor cells with an unequivocal positive reaction. Each section was independently assessed by 2 pathologists without prior knowledge of patient data. 免疫组织化学染色结果分配一个平均评分， 既考虑一个明确阳性反应染色强度和肿瘤细胞的 比例 Positive reactions were defined as those showing brown signals in the cell cytoplasm. For -catenin, a staining index (values, 0-12) was determined by multiplying the score for staining intensity with the score for positive area. 阳性反应被定义为那些细胞浆显示棕色信号的。

对于-catenin，一个染色指数（0-12）被 定义为染色强度和染色区域的乘积 The intensity was scored as follows: 0, negative; 1, weak; 2, moderate; and 3, strong. The frequency of positive cells was defined as follows: 0, less than 5%; 1, 5% to 25%; 2, 26% to 50%; 3, 51% to 75%; and 4, greater than 75%. 染色强度的分数被定为：阴性 0 分；弱 1 分；中等 2 分；强阳性 3 分阳性细胞的频率被定 义为：少于 5%，0 分；5%-25%，1 分；26%-50%，2 分；51%-75%，3 分；大于 75%，4 分 When the staining was heterogeneous, we scored it as follows: each component was scored independently and summed for the results. For example, a specimen containing 75% tumor cells with moderate intensity (3 2 =6) and another 25% tumor cells with weak intensity (1 1 = 1) received a final score of 6 + 1 = 7. 当染色异构的时候，我们这样评分 ： 每个评分都是独立的，结果是综合的。

比如说，一个标 本包含75%的肿瘤细胞中等强度染色 （3*2=6分） ， 另外的25%的肿瘤细胞是弱强度的 （1*1=1） 最后得到的结果是 6+1=7 For statistical analysis, scores of 0 to 7 were considered low expression and scores of 8 to 12 considered high expression. 数据分析的时候，0-7 被认为是低表达而 8 到 12 被认为是高表达。