BG11液体培养基.doc

BG11液体培养基BG11液体培养基就是：Stock 1 定容 100mL柠檬酸0.3g柠檬酸铁胺0.3gEDTANa2 0.05gStock2 定容 lOOOmLNaNO3 30gK2HPO4 0.78gMgSO4-7H2O 1.5gStock3 定容 lOOmLCaC12-2H2O 1.9gStock4 定容 lOOmLNa2CO3 2gStock5 定容 1 OOOmLH3BO3 2.86gMnC12-4H2O 1.81gZnSO4-7H2O 0.222gNa2MnO牛 2H2O 0.391gCuSO4-5H2O 0.079gCo(NO3)2・6H2O 0.049gStock 1 取用 2mLStock2 取用 20mLStock3 取用 2mLStock4 取用 ImLStock5 取用 ImL总定容1 OOOmLComponentAmountStock Solution⑴NaNO?100 mL/L15 0 g/L dH2O(2) K2HPO410 mUL2 g/500 mL dH-0⑶ MgSO4 7H2O10 mL/L3 75 g/500 mLdH.O(4 ) CaCI22H2O10 mUL1 8 g/500 mL dH?O(5 ) Citric acid10 mUL0 3 g/500 mL dH2O(6 )Ferric ammonium citrate10 mL/L0 3g/500ml dH?O(7 ) EDTANa,10 mL/L0 05g/500ml dH?O(8) Na2CO310 mL/L1 0g/500ml dH-0(9) A5 (Trace mental solution ) 1ml/Lh2bo22.86g/L dH-0MnCL4H,01 86g/L dH-0ZnSO47H.O0 22g/L dH-0Na.MoO 乙 2H.0v 4. v0 39g/L dH2OCuSO. 5 H.O4. z0 08g/L dH2OCo(N00)9.6 H,00 05g/L dH2OAdjust PH to 7.1 with 1M NaOH or HCID1 (Diatom medium)ComponentAmountStock Solution(1)NaNO31 mL/L12g/100mldH2O(2) K2HPO41 mL/L4 g/100 mLdH2O(3) MgSO4-7H2O1 mL/L7g/100 mLdH2O2.86g/L dH2O1.86g/LdH2O0.22g/L dH2O0.39g/L dH2O0.08g/L dH2O0.05g/L dH2O20 mL/L(4 ) CaCI2-2H2O1 mL/L2g/100mLdH2O(5 ) KH2PO41 mL/L8g/100 mLdH2O(6 ) Na2SiO3.9H2O1 mL/L10g/100mldH20⑺ MnSCU0.1 mL/L0.2g/100mldH20(8)柠檬酸铁1 mL/L0.5g/100mldH20(9) A5 (Trace mental solution)1ml/Lh3bo3MnCI24H2OZnSO47H2ONa2MoO4.2H2OCuSO4. 5 H20Co(NO3)2.6 H20(10) 土壤提取液 土壤提取液配制方法：取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸憾水 1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时, 冷却，沉淀24小时，此过程连续进行3次，然后过滤，取上清液，于高压灭菌锅中灭菌后于4C 冰箱中保存备用。

f/2 medium1000 mLComponent Amount⑴NaNO375 mg/L(2)NaH2PO4 ・ 2H2O0.6 mg/L⑶Vitamin B120.5 pg/L⑷Biotin0.5 pg/LThiamine HCI100|jg/LNa2SiO3 ・ 9H2O10 mg/L⑺f/2 metals1 mL/LNa2EDTA ・ 2H2O4.4gFeCI3 ・ 6H2O3.16 gCoSO4 ・ 7H2O0.012gZnSO4 ・ 7H2O0.021gMnCI2 ・ 4H2O0.18gCuSO4 ・ 5H2O0.007gNa2MoO4 ・ 2H2O0.007gDistilled water1000 mL(8) Seawater。