A1血液常规分析操作规程.docx

Al血液常规分析操作规程Procedures for routine blood routine analysis of AlAl.1 home pageProcedure for routine blood analysisTianjin HospitalOperating procedure file number: AlThird EditionA total of 6 pagesMarch 10, 2003 implementationThis procedure is reviewed every 2 yearsDate of review: March 10, 2009Retrial: Huang JinProgram editor: Huang JinApprover: Wang YiDate of approval: March 10, 2003Document distribution departments and / or individuals:A medical department; a custodian:Dean，s office; custodian:Hospital record room; custodian:Laboratory division: Director: Wang YiClinical laboratoryPrinciple of Al. 2 detectionSYSMEX-2000i blood cell analyzer, using resistance and fluorescence staining, flow cytometry principle counting white blood cells, red blood cells and platelets, and colorimetric method to determine hemoglobin・Al. 3 testing itemsThe thirty parameters of SYSMEX-2000i blood cell analyzer respectively: white blood cell (WBC), lymphocyte percentage and absolute value (LY%, LY), the percentage of mononuclear cells and the absolute value (MO%, MO), the percentage of neutroph订s (NEUT%, NEUT), eosinophils (EO%, EO, No.), basophils (BA%, BA), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell volume distribution width standard deviation (RDW-SD), red blood cell volume distribution width coefficient of variation (RDW-CV), platelet (PLT), platelet volume distribution width (PDW), plateletcrit (PCT), mean platelet volume (MPV), Large platelet ratio (P-LCR), reticulocyte percentage (RET%) and reticulocyte (RET), the absolute value of the low fluorescence intensity of reticulocyte percentage (LFR), the fluorescence intensity of the reticulocyte percentage (MFR), high fluorescent reticulocyte percentage (HFR), immature reticulocyte percentage (IRF)・A1.4 specimen collection and processingSYSMEX~2000i blood cell analyzer with EDTA anticoagula.nt venous blood for blood analysis・Venous blood collection: the blood is usually taken from the cubital vein, and infants can take blood from the jugular and femoral veins・ The use of tourniquet time not more than 1 minutes, puncture immediately after the successful release of the tourniquet, when need to use tourniquet should be another arm・(1) blood sampling apparatus: the venous blood sampling apparatus adopts a lattice vacuum collecting blood vessel and a 7 aseptic needle matched with it.(2) the operation steps of blood collection: operation according to standardized operation rules of blood sampling・ After the blood is collected, gently turn the vacuum collection vessel into reverse and mix it 8 to 10 times to prevent blood clots or platelet emboli・Al.5 reagentAl. 5. 1 the laboratory uses a kit produced by SYSMEX, Japan, which includes diluents, hemolytic agents, and detergents・ In addition, the company also produces quality control products and correction fluid.(1) dilution CELLPACK (PK-30L), number 88408711(2) 白细胞分类溶血素 stromatolyser 4dl (ffd),编号 98417615(3) 白细胞分类染液 stromatolyser 4ds (ffs),编号 98417216(4) 嗜碱细胞溶血素 stromatolyser (fb, fba 一 200a 编号 94404516)(5) 血红蛋白溶血素 sulfolyser (sis 210a),编号 90411317(6) 网织红细胞溶血素ret search ii delution (700a,编号 98416211 red)(7) 网织红细胞染液 ret search ii (red stain,编号 98416211 700a)(8) 清洗液 cellclean (cl 编号 83401621 50)(9) 配套质控品 e check level 1、e check level 2、e check level 3.al. 6仪器操作 al. 6. 1开机前检查废液桶是否充满废液，并及时倾倒.al. 6. 2打开打印机电源开关.al. 6. 3打开电脑：al. 6. 3. 1打开电脑显示器电源开关.al. 6. 3. 2打开电脑主机电源开关.al. 6. 3. 3仪器操作系统自动启动后，在弹出的ipu登录对话框中输 入用户名和密码，然后点击【确定】・（此时即可打开xt - 20001的 主机电源开关・）al.6. 3. 4双击桌面上中文软件图标【血球仪xt - 2000i】，并登录 （输入操作者的用户名和密码）.al. 6. 4 打开 xt 一 2000i 主机：al.6. 4. 1打开xt - 20001主机电源开关（此步骤已在前面完成）.:*注意岀现问题时，千万不要在短时间内反复开关电源，至少要 间隔30秒钟以上，否则极易损坏cpu板.al. 6. 4. 2开机后仪器开始自动冲洗及本底检查，如果木底在允许范 围以内，仪器进入待机状态.*如果本底太高，将岀现空白错误提示•需要在登录完成后，再次进 行自动冲洗.al. 6. 4.3当xt - 2000i操作界面最下方仪器状态图标变为绿色，且 主机手动进样针上方绿色指示灯亮起，即可进行常规操作.:*注意如果10分钟后，机器没有进行任何操作，则压缩机将自动 转入休眠状态，以延长寿命和减少噪声•此时，只要按一下仪器止面 右下方绿色的【start]键（手工进样键），就可以回到正常的待机状 态.al. 6. 5手动方式分析样本：al. 6. 5. 1点击仪器操作软件上的【手动】键，进入手工吸引方式.al. 6. 5. 2用数字键输入标本号.al. 6. 5.3从【随选】菜单中选择所需要的分析项冃，【方式】为手 工.al. 6. 5.4将标本充分颠倒混匀10次，取下管帽，置于手工吸样针下 方，按一下绿色的【start] JT关，通过吸样针吸入标本•稍过片刻， 分析数据会送到电脑.al. 6. 6自动进样方式分析样本：注：真空采血管盖帽必须盖牢盖正，不然会引起穿刺针的偏移.al. 6. 6.1按下主机面板上的【进样器】键，The settings menu appears on the screen.Al.6.6.2 input [sample number], [pipe rack number] (generally do not lose), [test tube position], select test project portfolio [Discrete]•Al. 6. 6. 3 will test tubes in order according to the number, click [start injector] key, in the subsequent confirmation screen [press] key, the instrument will automatically complete batch testing.Al. 6. 6. 4 at this point in Chinese software [testing] menu and save the patient information input, (this step can also be carried out in the analysis, before or after an。