DNA固定在纳米颗粒表面的方法.ppt

磷酸化的DNABiotin-DNA炔基修饰的DNA巯基修饰的DNA氨基修饰的DNA金银纳米颗粒碳纳米管、石墨烯二氧化硅纳米颗粒量子点上转换纳米颗粒磷酸化的DNA与TiO2π-π堆积，如单链DNA与石墨烯，碳纳米管静电吸附1、Biotin-DNA2、炔基修饰的DNA及其他生物分子3、NH2-DNA氨基 羧基氨基 羧基氨基 氨基1〕EDC和NHSFor best results in two-step reactions, perform the first reaction in MES buffer (or other non-amine, non-carboxylate buffer) at pH 5-6, then raise the pH to 7.2-7.5 with phosphate buffer (or other non-amine buffer) immediately before reaction to the amine-containing molecule氨基 羧基DCC〔N,N′-Dicyclohexylcarbodiimide〕氨基-氨基1〕BS(PEG)n PEG Crosslinkers - Amine Reactive 2〕TSAT (Tris-succinimidyl aminotriacetate)WaterWaterNHS esters:pH 7-93〕DSS (disuccinimidyl suberate)4〕BSOCOES (bis[2-(succinimidyloxycarbonyloxy)ethyl]sulfone) 5〕DSG (disuccinimidyl glutarate)6〕DTSSP (3,3´-Dithiobis[sulfosuccinimidylpropionate]) WaterWaterWaterDMSO or DMF8〕BS³ (Bis[sulfosuccinimidyl] suberate) 9〕EGS [ethylene glycolbis(succinimidylsuccinate)] 10〕Sulfo-EGS [ethylene glycolbis(sulfosuccinimidylsuccinate)]7〕DST (disuccinimidyl tartrate)WaterWaterDMSO or DMFDMSO or DMF4、巯基化的DNA1 Au-S Ag-S2 巯基 氨基3 巯基 羧基4 巯基 羟基5 巯基 巯基巯基-氨基1〕AMAS (N-[α-maleimidoacetoxy] succinimide ester)NHS esters:pH 7-9；；。

Two steps reactionDMSO or DMF2〕BMPS (N-[β-maleimidopropyloxy] succinimide ester)DMSO or DMF3〕EMCS (N-[ε-maleimidocaproyloxy]succinimide ester)DMSO or DMF4〕Sulfo-EMCS (N-[ε-maleimidocaproyloxy]sulfosuccinimide ester)Water6〕Sulfo-GMBS (N-[γ-maleimidobutyryloxy]sulfosuccinimide ester)5〕GMBS (N-[γ-maleimidobutyryloxy]succinimide ester)DMSO or DMFWater7〕BMPA (N-β-maleimidopropionic acid)EDC，，8〕LC-SMCC (Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate])DMSO or DMF10〕Sulfo-SMPB (sulfosuccinimidyl 4-[p-maleimidophenyl]butyrate)9〕SMPB (succinimidyl 4-[p-maleimidophenyl]butyrate)DMSO or DMFWater11〕Sulfo-LC-SPDP, LC-SPDP, SPDP巯基-羧基巯基-羟基异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。

如在水相中，那么要使用pH8.5的碱性缓冲溶液，防止使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH＞7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯基发生反响巯基-巯基表征：1、定性〔红外表征，如交联剂的特征峰；测颗粒的在260纳米处的紫外吸收，跑电泳，电位，粒径变化〕2、定量〔测颗粒或上清的紫外吸收，荧光，热重力分析〕。