荧光染料激发和发射光谱表.doc

荧光素名称 激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA) 542 568 5-FAM (5-Carboxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-540 5-Hydroxy Tryptamine (HAT) 370-415 520-540 5-ROX (carboxy-X-rhodamine) 578567 604591 5-TAMRA (5-Carboxytetramethylrhodamine)548542 552568 6-Carboxyrhodamine 6G 518 543 6-CR 6G 518 543 6-JOE 520 548 7-Amino-4-methylcoumarin 351 430 7-Aminoactinomycin D (7-AAD) 546 647 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 540 630 ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474 Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Orange, both DNA & RNA 440-480 520-650 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 Acriflavin Feulgen SITSA 355-425 460 Aequorin (Photoprotein) 466 PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350™ 346342 442441 Alexa Fluor 430™ 431 540 Alexa Fluor 488™ 495, 492 519, 520 Alexa Fluor 532™ 531, 532 553, 554 Alexa Fluor 546™ 556, 557 572, 573 Alexa Fluor 568™ 577, 578 603 Alexa Fluor 594™ 590, 594 617, 618 Alexa Fluor 633™ 632 650 Alexa Fluor 647™ 647 666 Alexa Fluor 660™ 668 698 Alexa Fluor 680™ 679 702 Alizarin Complexon530-560, 580580, 624-645Alizarin Red 530-560 580 Allophycocyanin (APC) 630, 645 655, 660 AMC, AMCA-S 345 445 AMCA (Aminomethylcoumarin) 345347 425444 AMCA-X 353 442 Aminoactinomycin D 555 655 Aminocoumarin 346350 442445 Aminomethylcoumarin (AMCA) 345347 425444 Anilin Blue 600 Anthrocyl stearate 360-381 446 APC (Allophycocyanin) 630, 645 655, 660 APC-Cy7 625-650 755 APTRA-BTC = Ratio Dye, Zn2+ 466/380 520/530 Ratio Dye, Zn2+APTS 424 505 Astrazon Brilliant Red 4G 500 585 Astrazon Orange R 470 540 Astrazon Red 6B 520 595 Astrazon Yellow 7 GLL 450 480 Atabrine 436 490 ATTO-TAG™ CBQCA 465 560 ATTO-TAG™ FQ 486 591 Auramine 460 550 Aurophosphine G 450 580 Aurophosphine 450-490 515 BAO 9 (Bisaminophenyloxadiazole) 365 395 BCECF (high pH) 492, 503 520,528 BCECF (low pH) 482 520 Berberine Sulphate 430 550 Beta Lactamase 409 447, 520 BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRET Bimane 398 490 Bisbenzamide 360 461 Bisbenzimide (Hoechst) 360 461 bis-BTC = Ratio Dye, Zn2+ 455/405 529/505 Ratio Dye, Zn2+Blancophor FFG 390 470 Blancophor SV 370 435 BOBO™ -1 462 481 BOBO™ -3 570 602 Bodipy 492/515 490 515 Bodipy 493/503 533 549 Bodipy 500/510 509 515 Bodipy 505/515 502 510 Bodipy 530/550 528 547 Bodipy 542/563 543 563 Bodipy 558/568 558 569 Bodipy 564/570 564 570 Bodipy 576/589 579 590 Bodipy 581/591 584 592 Bodipy 630/650-X 625 642 Bodipy 650/665-X 647 665 Bodipy 665/676 605 676 Bodipy Fl 504, 505 511, 513 Bodipy FL ATP 505 514 Bodipy Fl-Ceramide 505 511 Bodipy R6G SE 528 547 Bodipy TMR 542 574 Bodipy TMR-X conjugate 544 573 Bodipy TMR-X, SE 544 570 Bodipy TR 589 617 Bodipy TR ATP 591 620 Bodipy TR-X SE 588 616 BO-PRO™ -1 462 481 BO-PRO™ -3 544 570 Brilliant Sulphoflavin FF 430 520 BTC - Ratio Dye Ca2+ 464/401 533/529 Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+ 459/417 517/532 Ratio Dye, Zn2+Calcein 494 517 Calcein Blue 373 440 Calcium Crimson™ 588, 589 611, 615 Calcium Green 501, 506 531 Calcium Green-1 Ca2+ Dye 506 531 Ca2+ DyeCalcium Green-2 Ca2+ 506/503 536 Ca2+Calcium Green-5N Ca2+ 506 532 Ca2+Calcium Green-C18 Ca2+ 509 530 Ca2+Calcium Orange 549 575576 Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX) 576 601 Cascade Blue™377398399 420423 Cascade Yellow 399400 550552 Catecholamine 410 470 CCF2 (GeneBlazer) CFDA 494 520 CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRET Chlorophyll 480 650 Chromomycin A 436-460 470 Chromomycin A 445 575 CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pHCMFDA 494 520 Coelenterazine Ca2+ Dye, bioluminescence (429) 465Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+ Dye, bioluminescenceCoelenterazine f (437) 473 Ca2+ Dye, bioluminescenceCoelenterazine fcp 452 Ca2+ Dye, bioluminescenceCoelenterazine h (437) 464 Ca2+ Dye, bioluminescenceCoelenterazine hcp (433) 444 Ca2+ Dye, bioluminescenceCoelenterazine ip 441 Ca2+ Dye, bioluminescenceCoelenterazine n (431) 467 Ca2+ Dye, bioluminescenceCoelenterazine O 460 575 Coumarin Phalloidin 387 470 C-phycocyanine CPM Methylcoumarin 384 469 MethylcoumarinCTC 400-450 602 CTC Formazan Cy2™ 489 506 Cy3.1 8 554 568 Cy3.5™ 581 598 Cy3™514552554 566570 Cy5.1 8 649 666 Cy5.5™ 675 695 Cy5™ 649 666670 Cy7™ 710, 743 767, 805 Cyan GFP 433。