BOD5测定仪(中英文说明二).doc

BOD5测测定定仪仪使使 用用 安安 装装 说说 明明 （二）泰州市奥普特分析仪器有限公司TaiZhou City AoPuTe Analysis Instruments Co. Ltd.VI, analytical methods and procedures (A) preparation, four inorganic salts Take 4 1000 ml Clean volumetric flask set aside. 1、 Buffer solutionDissolving the following reagent with distilled water and dilute to 1000 ml (1) 、21.75 g Potassium phosphate, dibasic (k2HPO4) (2) 、8.5 g Potassium dihydrogen phosphate (KH2PO4) (3) 、33.4 g Sodium phosphate, dibasic ( N a 2 HPO 4 · 7 H 2 O Humidity in the 61.0 7 %~80.51% stable) (4) 、1.7 g Ammonium chloride ( NH 4 Cl ） 2 And magnesium sulfate solution Dissolved in distilled water 22.5 g Magnesium sulfate ( MgSO 4 ·7H 2 O ) And dilute to 1000 ml. 3 And calcium chloride solution Dissolved in distilled water is 27.5 g Calcium chloride ( CaCl 2 ) And dilute to 1000 ml. 4 And ferric chloride solution Dissolved in distilled water 0.25 g Ferric chloride ( FeCl 3 ·6H 2 O ) And dilute to 1000 ml 。

(B) sample BOD Measurement of the 1 , Anticipating specimens which are to be BOD A range of values, select the close range. If unknown, the samples can be COD value, and thereby determine the range (usually samples of BOD values for samples of the COD value of 0.8 times. BOD 1000mg/L , microbial samples containing sufficient oxygen, without vaccination, either directly according to the selected measuring range, found samples from the sampling scale weight. Depending on the number of test samples (up to 8 ) to determine the one samples a few flasks. If there are only two samples, you can choose 2~4 determination of flasks, one water sample. Estimates of the sample BOD value range, determine the amount of sampling each bottle to determine several flasks of required total amount of sampling. Place the water in a large beaker (1000ml~2000ml), per 1000ml Joined four inorganic salts the water sample 1ml. Put the beaker into the incubator Vice either experiment on location mixing temperature of 2~3 hours. Both must adjust the water's pH value 6. 7 ~7.5 (the sweet spot pH7.2 ）。

Outside this range, use appropriate concentrations of sodium hydroxide or sulphuric acid neutralization. Cylinder sampling by fixed measure sampling poured into the flask.Sampling scales (according to flask volume to fill in the actual values from the factory) Measuring range (mg/l)0 ～ 200 ～1000 ～3000 ～5000 ～8000 ～1000Taking samples 2、 Placed in each flask 1 mixed child, flasks placed on the Deputy's experiment, note, flasks and Vice- Match the channel number is indicated on, ensure that the set value, the measured value and the exact correspondence between the actual samples. Heat stirring until the water temperature reaches 20℃ ±1℃ (approximately 1~2 hours). 3、 Take 8 cleaning clean the sealing Cup, Cup, puts the total height is about 1/4 solid NaOH KOH ( Or 5~ 6 Particle size NaOH Particle ), sealed glass bottle mouth and the head has two connections with the surface coated with a thin layer of grease in vacuum, and then placed in each bottle. With hose connection CAP in flasks, rotated through tight, while Deputy machine 8reference pressure cartridge seals only tighten bolts, must not allow this process NaOH or KOH into the culture fluid. 4、 As previously mentioned, reattached after a check on clock, timing, range set value is accurate without False positives. If you do not need vaccinations, vaccination should be set value is set to 0, this is the mode of non-vaccination,1~8 -channel direct measurements of all as samples. Inoculation when set value is 0, the definition for inoculation model, it will inevitably cause computational errors. 5 And inoculated settings. Some lack of samples of micro-organisms require inoculation (specific operations see “inoculation“ section), requires that all samples of vaccine rate , and require the inoculated water samples while conducting parallel experiments, inoculation and water must be placed on 1 channel. As the vaccine rate, sample for 10%, pressing the key inoculation, enter the numeric key 1, and0, then press the Enter key to confirm, the display appears “End“. Reset and then vaccination key to confirm and correct. 6 , And thus can be reset by pressing the start key, start experimenting until the set time after the completion, the instrument automatically terminate data collection and displayed in the time display window “ End “Words, internal buzzer beep prompt end of the experiment. 7 , And then press the clear key to end buzzer beep, then press the print button, you can print a single channel or all channels Complete biochemical reaction curves or data. 8 、 Equipment is protected from power failure, in the process, in case of a sudden power outage, all data will be lost. Call display window displays briefly, “run“, the instrument automatically resumes without the need for human intervention (power outage time may affect the measurement result). 9 , Apparatus, once started, may not be hitting the reset button. As a result of special circ。