（精编资料推荐）常用酶切位点表(含保护碱基).doc

……………………………………………………………专业资料推荐…………………………………………………酶 寡核苷酸序列 切割率% 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 >90 >90 0 >90 >90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA >90 >90 >90 >90 >90 >90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 >90 >90 >90 >90 >90 BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 >90 >90 25 >90 >90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 >90 >90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0 >90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 >90 Cla I CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG 0 0 >90 50 0 0 >90 50 EcoR I GGAATTCC CGGAATTCCG CCGGAATTCCGG >90 >90 >90 >90 >90 >90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA >90 >90 >90 >90 >90 >90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 >90 >90 0 >90 >90 Mlu I GACGCGTC CGACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGGCATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 >90 >90 Nhe I GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 酶寡核苷酸序列切割率%2 hr20 hrNot ITTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA0 10 10 25 250 10 10 90 >90Nsi ITGCATGCATGCA CCAATGCATTGGTTCTGCAGTT10 >90>90 >90Pac ITTAATTAA GTTAATTAAC CCTTAATTAAGG0 0 00 25 >90Pme IGTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG0 0 0 750 25 50 >90Pst IGCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT0 10 >90 >90 00 10 >90 >90 0Pvu ICCGATCGG ATCGATCGAT TCGCGATCGCGA0 10 00 25 10Sac ICGAGCTCG1010Sac IIGCCGCGGC TCCCCGCGGGGA0 500 >90Sal IGTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0 10 100 50 75Sca IGAGTACTC AAAAGTACTTTT10 7525 75Sma ICCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA0 0 10 >9010 10 50 >90Spe IGACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG10 10 0 0>90 >90 50 50Sph IGGCATGCC CATGCATGCATG ACATGCATGCATGT0 0 100 25 50Stu IAAGGCCTT GAAGGCCTTC AAAAGGCCTTTT>90 >90 >90>90 >90 >90Xba ICTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG0 >90 75 750 >90 >90 >90Xho ICCTCGAGG CCCTCGAGGG CCGCTCGAGCGG0 10 100 25 75Xma ICCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA0 25 50 >900 75 >90 >904。