隔药饼灸对功能性胃肠病肝郁脾虚模型大鼠胃肠激素及胃肠动力的影响.pdf

2013-04-18|Volume 21|Issue 11|WCJD|刘未艾, 湖南中医药大学第二附属医院针灸脑病科 湖南省长沙市 410005郁保生, 湖南中医药大学中医学院 湖南省长沙市 410007常小荣, 刘密, 岳增辉, 湖南中医药大学针灸推拿学院 湖南省长沙市 410007章海凤, 湖南中医药大学 湖南省长沙市 410007 刘未艾, 在读博士, 副主任医师, 主要从事针灸治病机制的研究.国家973计划理论专项课题基金资助项目, No. 2009CB522904国家自然科学基金课题基金资助项目, Nos. 81173326, 30973802 长沙市科技计划重点基金资助项目, No. K1005020-31湖南省高校创新平台开放基金资助项目, No.12K087高等学校博士学科点专项科研基金资助项目, No. 20124323110001湖南省科技计划基金资助项目, No. 2011SK3095作者贡献分布: 此课题由郁保生、常小荣与刘密设计; 动物模型制作和指标检测分析由刘未艾、章海凤及岳增辉完成; 论文写作由刘未艾与常小荣完成.通讯作者: 郁保生, 教授, 410007, 湖南省长沙市韶山中路113号, 湖南中医药大学针灸推拿学院. ybs1955@: 0731-88458072收稿日期: 2012-08-22 修回日期: 2013-01-17接受日期: 2013-03-21 出版日期: 2013-04-18Influence of herbal-cake-separated-moxibustion on gastrointestinal hormones and gastrointestinal motility in rats with functional gastrointestinal disorders and syndrome of liver stagnation and spleen deficiencyWei-Ai Liu, Bao-Sheng Yu, Xiao-Rong Chang, Mi Liu, Hai-Feng Zhang, Zeng-Hui YueWei-Ai Liu, Department of Acupuncture-Encephalopathy, the Second Affiliated Hospital of Hunan University of Tra-ditional Chinese Medicine, Changsha 410005, Hunan Prov-ince, ChinaBao-Sheng Yu, the College of Traditional Chinese Medi-cine, Hunan University of TCM, Changsha 410007, Hunan Province, ChinaXiao-Rong Chang, Mi Liu, Zeng-Hui Yue, College of Acu-moxibustion and Massage, Hunan University of Tradi-tional Chinese Medicine, Changsha 410007, Hunan Prov-ince, ChinaHai-Feng Zhang, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, ChinaSupported by: the National Key Basic Research Pro-gram (“973” Program), No. 2009CB52290; the National Natural Science Foundation of China, Nos. 81173326 and 30973802; the Key Science and Technology Program of wcjd@ 世界华人消化杂志 2013年 4月 18日 ; 21(11): 1002-1007ISSN 1009-3079 (print) ISSN 2219-2859 (online)研究快报 RAPID COMMUNICATION隔药饼灸对功能性胃肠病肝郁脾虚模型大鼠胃肠激素及胃肠动力的影响刘未艾, 郁保生, 常小荣, 刘 密, 章海凤, 岳增辉®■ 背景资料功 能 性 胃 肠 病(FGIDs)是 消 化 内科 常 见 的 疾 病 , 该病 的 病 因 和 发 病机 制 多 考 虑 与 精神 心 理 因 素 和 胃肠 动 力 障 碍 有 关 , 目 前 西 医 采 取 对症 治 疗 , 无 特 效 治疗 方 案 , 而 灸 法 作为 治 疗 FGIDs的优 势 病 种 获 得 满意 的 疗 效 . Changsha, No. K1005020-31; the Hunan Provincial Colleg-es and Universities Innovation Platform Open Fund Project, No. 12K087; the Higher School Specialized Research Fund for the Doctoral Program, No. 20124323110001; the Hunan Science and Technology Program, No. 2011SK3095Correspondence to: Bao-Sheng Yu, Professor, College of Traditional Chinese Medicine, Hunan University of TCM, 113 Shaoshan Middle Road, Changsha 410007, Hunan Province, China. ybs1955@ Received: 2012-08-22 Revised: 2013-01-17Accepted: 2013-03-21 Published online: 2013-04-18AbstractAIM: To observe the influence of herbal-cake-separated moxibustion on gastric emptying, small intestinal transition and gastrointestinal hormones in rats with functional gastrointestinal disorders due to syndrome of liver stagnation and spleen deficiency and to explore the possible mechanism underlying the therapeutic effects of herbal-cake-separated-moxibustion against functional gastrointestinal disorders. METHODS: Sixty SD rats were randomly and equally divided into five groups: blank group, model group, herbal-cake-separated-moxibus-tion group, Xiaoyaosan group and domperidone group. Except for the blank group, the other four groups underwent modeling by applying chron-ic restraint stress + excessive fatigue + irregular food for 21 consecutive days. The herbal-cake-separated-moxibustion group underwent herbal cake separated moxibustion for 14 d, while the Xiaoyaosan group and domperidone group were given Xiaoyaosan and domperidone by ga-vage for the same duration, respectively. At the end of the experiment, gastric emptying rate and small intestinal transit rate were determined, and serum levels of motilin (MTL), gastrin (GAS) and vasoactive intestinal peptide (VIP) were measured by enzyme-linked immunosorbent as-say.RESULTS: Serum VIP level and gastric empty-ing rate were significantly lower in the herbal-cake-separated-moxibustion group, Xiaoyaosan group and domperidone group than in the mod-■ 同行评议者郑 培 永 , 副 教 授 , 上 海 中 医 药 大 学脾 胃 病 研 究 所 , 上海 中 医 药 大 学 附属 龙 华 医 院 消 化内 科刘未艾, 等. 隔药饼灸对功能性胃肠病肝郁脾虚模型大鼠胃肠激素及胃肠动力的影响 10032013-04-18|Volume 21|Issue 11|WCJD|■ 研发前沿目 前 针 对 灸 法 治疗 FGIDs的 临 床研 究 较 多 , 通 过 动物 实 验 研 究 其 机制 的 报 道 较 少 , 因此 对 灸 法 的 疗 效缺 少 客 观 公 认 的评 价 . el group (VIP: 155.71 pg/mL ± 14.66 pg/mL, 151.79 pg/mL ± 11.46 pg/mL, 147.03 pg/mL ± 22.16 pg/mL vs 170.55 pg/mL ± 16.83 pg/mL, all P 0.05).CONCLUSION: Herbal cake-separated-moxi-bustion can promote gastric emptying and small intestinal transit possibly by increasing serum levels of MTL and GAS and reducing serum level of VIP. © 2013 Baishideng. All rights reserved.Key Words: Herbal-cake-separated-moxibustion; Functional gastrointestinal disorders; Motilin; Gastrin; Vasoactive intestinal peptide; Gastric emptying; Small intestinal transitLiu WA, Yu BS, Chang XR, Liu M, Zhang HF, Yue ZH. Influence of herbal-cake。