标准缓冲液pH值与温度对照表.doc

标准缓冲液pH值与温度对照表温度C四草酸氢钾0.05M邻苯二甲酸氢钾0.05M混合磷酸盐0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(0.05mol/L )X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07 , 0.2 mol/L甘氨酸溶液含15.01克/升2.邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾+ 0.2 mol/L HCl，再加水稀释到20毫升pH(20C)XYpH(20 C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量 =204.23 , 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3.磷酸氢二钠-柠檬酸缓冲液pH0.2 mol/LNaHPO（毫升）0.1 mol/L柠檬酸（毫升）pH0.2 mol/LNaHPO（毫升）0.1 mol/L柠檬酸（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.24.44.64.85.08.288.829.359.8610.3011.7211.1810.6510.149.707.27.47.67.88.017.3918.1718.7319.1519.452.611.831.270.850.55NaHP时子量=14.98 , 0.2 mol/L 溶液为 28.40 克/升。

NaHPQ2H2分子量=178.05 , 0.2 mol/L 溶液含 35.01 克/升CHQ・ I4O分子量=210.14 , 0.1 mol/L 溶液为 21.01 克/ 升4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L ）柠檬酸（克）C6F8O - H2O氢氧化钠（克）NaOH 97%盐酸（星升）HCl （浓）最终体积（升）①2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.4528518610510106.50.38266156126① 使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢 氧化钠溶液或浓盐酸调节，冰箱保存5.柠檬酸-柠檬酸钠缓冲液 （0.1 mol/L ）pH0.1 mol/L0.1 mol/LpH0.1 mol/L0.1 mol/L柠檬酸柠檬酸钠柠檬酸柠檬酸钠（毫升）（毫升）（毫升）（毫升）3.018.6「1.45.08.211.82.83.217.24.05.27.312.73.416.05.15.46.413.63.614.96.05.65.514.53.814.06.95.84.715.34.013.17.76.03.816.24.212.38.66.22.817.24.411.49.76.42.018.04.610.310.86.61.418.64.89.2柠檬酸GHO - HO:分子量210.14 , 0.1 mol/L 溶液为21.01克/升。

柠檬酸钠Na CeHfeO - 2H2O:分子量294.12 , 0.1 mol/L 溶液为29.41克/毫升6.乙酸-乙酸钠缓冲液(0.2 mol/L )pH（18C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc佬升）2.60.759.253.81.208.804.01.808.204.22.657.354.43.706.304.64.905.10pH（18 C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc（毫升）4.85.904.105.07.003.005.27.902.105.48.601.405.69.100.905.89.400.60NaAc・ 3RO分子量=136.09 , 0.2 mol/L 溶液为 27.22 克/ 升7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)pH0.2 mol/L Na2HPO（毫升）0.3 mol/L NaHPO（毫升）pH0.2 mol/LNaHPO（毫升）0.3 mol/LNaH2PO（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.86.949.555.051.045.08.094.75.3溶液为85.61克/升NaHPQ・2HO分子量NaHPQ・2HO分子量NaHPO・ 2H2O分子量=178.05 , 0.2 mol/L=358.22 , 0.2 mol/L=156.03 , 0.2 mol/L溶液为71.64克/升溶液为31.21克/升（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15 mol/L ）P，mol/L/15Na2HPmol/L//15KH 2PF)mol/L//15Na 2HPmol/L//15KH 2PHQOH（毫升）（毫升）（毫升）（毫升）40.109.90' 7.003.00.92.170.509.508.002.005.291.009.00.389.001.005. 2.008.00， 9.500.50.91.733.007.009.750.256i8.244.006.00.049.900.106i 5.005.008j 10.000.47.346.004.006i8.64.676i8.81.186i.98NaHPO・ 2I4O分子量=178.05 , 1/15M 溶液为 11.876 克/ 升。

KHPO分子量=136.09 , 1/15M溶液为9.078克/升8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO + Y毫升0.2N NaOH加水稀释至29毫升pH(20C)X （毫升）Y （毫升）pH(20 C)X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）6.810018.48.41005.21 [7.010017.88.61003.827.210016.78.81002.527.410015.39.01001.6510013.47.69.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10. Tris -盐酸缓冲液(0.05M, 25C)5。