组培文献翻译.docx

1、EFFECTS OF THE GROWTH RETARDANT PACLOBUTRAZOL ON LARGE-SCALEMICROPROPAGATION OF DAYLILY (HEMEROCALLIS SPP.)JIANXIN CHEN*, DAWN E. HALL, AND^CENZO DE LUCA大规模生长延缓剂多效唑影响黄花菜快繁(萱草SPP建新陈*,黎明E.霍尔和Vincenzo德卢卡Department of Biology, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario L 2S 3A1, Canada(Received 6 April 2004; accepted 5 September 2004; editor R. D. Shillito)格伦里奇大道500号，圣凯瑟琳，安大略省2S3A1,加拿大布鲁克大学，生物 系，(2004年4月接受2004年9月编辑RD希利托)SummaryMeristematic clusters were induced from daylily scape explants (pedicel -cape junction) in the presence of the growthretardant Paclobutrazol on semisolid agar medium. Liquid shake culture was used to proliferate meristematic clusters.Highly efficient regeneration of adventitious shoots occurred on clusters after subculture on a 0.8% agar strength semisolidmedium with the addition of activated charcoal. Paclobutrazol and sucrose levels in the media were found to significantlyaffect starch accumulation, growth value, and dry weight percentage of liquid-cultured meristematic clusters. The use ofliquid shake cultures for mass proliferation of meristematic clusters followed by regeneration of adventitiousshoots onsemisolid agar culture could be an efficient system for large-scale micropropagation of daylily.总结根尖诱导集群从黄花菜景观植(花梗景观路口)半固体琼脂培养基上生长延缓剂 多效唑的存在。

液体振荡培养物用于增殖分生集群高效再生不定芽发生在0.8%的琼脂强度与另外活性炭半固体培养基继代集群 多效唑和蔗糖水平在媒体上被发现显着影响淀粉的积累，成长值，液体分生组织 培养集群干重量百分比液体振荡培养大量增殖半固体琼脂培养不定芽再生的分 生组织集群使用可能是一个有效的黄花菜进行大规模快繁体系Key words: daylily; Hemerocallis; growth retardant; liquid culture; micropropagation. 关键词：黄花菜，黄花菜生长延缓剂，液体培养快繁技术Spent medium analysis for liquid culture micropropagationof Hemerocallis on Murashige and Skoog medium分析了介质为液体培养快速繁殖的萱草的Murashige和Skoog介质Jeffrey W・ Adelberg & Maria P. Delgado &Jeffery T. TomkinsReceived: 14 July 2008 / Accepted: 20 August 2009 / Editor: T. J. Jones# The Society for In Vitro Biology 2009杰弗里• Adelberg玛丽亚• P.德尔加多&杰弗里「汤姆金斯收稿日期：2008年7月14日/接受日期：2009年8月20日/编辑：TJ琼斯 2009年在体外生物学学会#Abstract Residual nutrients from Murashige and Skoog medium were analyzed following a 5-wk multifactor experiment. Plant density, sugar concentration, and plant growth regulators (benzyladenine and ancymidol) were examined using four genotypes of daylily (Hemerocallis) to determine which factors most influenced nutrient use. Active nutrient uptake was observed for 11 nutrients (potassium, sodium, copper, phosphorus, iron, calcium, magnesium, manganese, boron, sulfur, and zinc) with lower concentrations in spent medium than in the tissue water volume (fresh-dry mass expressed as mL HQ). Two patterns of nutrient use were visualized by correlative analysis of nutrient uptake. Greatest growth lowered plant nutrient concentrations of potassium, sodium, phosphorus, iron, and copper in all genotypes, and luxuriant uptake was indicated with least growth. Potassium, sodium, iron, and copper concentrations in plant dry matter were equal to or exceeded what is observed in vigorously growing nursery plants. However, phosphorus concentration in plant dry matter was low enough to be considered deficient when compared to Hemerocallis plants in nursery production. With a second group of nutrients (calcium, magnesium, manganese, and boron), the genotype, “Barbara Mitchell"lacked active uptake and was deficient. Calcium concentrationwas low in all plants compared to Hemerocallisgrown under nursery conditions （“Barbara Mitchell" was the lowest concentration） despite active uptake by the otherthree genotypes—“Brocaded Gown," “Mary's Gold," and“Heart of a Missionary." Magnesium concentration in thesethree genotypes was low enough in vessels with greatest growth to question its adequacy at high densities. Increased sucrose in medium reduced the dry matter concentrations of all tested nutrients. Plant growth regulators had less impact on nutrient use than genotype and plant density. Nutrient uptake may be an important physiological component of genotypic variation.摘要：从Murashige和SKOOG的残留养分介质中进行分析后，5周多因素的实 验。

种植密度，糖浓度，并使用4种基因型金针（黄花菜）检查，以确定哪些因 素最影响养分利用植物生长调节剂（苄基嘌吟和嘧啶醇）有效的营养物质的 吸收，观察11营养素（钾，钠，铜，磷，铁，钙，镁，锰，硼，硫，和锌）与较 低用过的培养基中的浓度比组织中的水的体积（mL水鲜干质量表示）相关 分析养分吸收的养分利用可视化两种模式最大的增长降低了所有基因型植物养 分浓度的钾，钠，磷，铁，铜，表示华丽的摄取至少增长在植物的干物质的钾， 钠，铁，铜的浓度分别为等于或超过所观察到的蓬勃发展苗木然而，磷浓度的 植物干物质低到足以被认为是比较缺乏萱草属植物育苗生产营养素（钙，镁， 锰和硼）的第二组，基因型，“巴巴拉米切尔”缺乏活性的吸收，是有缺陷的 在所有的植物相比，育苗条件下种植的萱草（“芭芭拉•米切尔”的最低浓度）， 尽管积极吸收其他钙离子浓度低三种基因型“锦缎袍”，“玛丽的黄金”和“心的传教士在这三种基因型镁浓度足够低增长最大的船只，质疑其在高 密度的充足增加培养基中的蔗糖的干物质浓度降低所有测试的营养素植物生 长调节剂比基因型和种植密度对养分利用影响较小营养物质的吸收，可能是一 个重要的生理组分的基因型变异Keywords Correlative analysis . Genotypic variation .Media formulation . Multifactor . Nutrients关键词：相关性分析、基因型差异、 媒体制定、多因素、养分3、4、SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROMCALLUS CULTURES OF SEVERAL SPECIES IN THE GENUS TRICYRTIS体细胞胚胎发生与植株再生愈伤组织培养几个种类在tricyrtis属MASARU NAKANO]*, KEIKO MIZUNASHI, SHIGEFUMI TANAKA^ TOSHINARI GODO2, MASASHI NAKATA2, and HIROYUKI SAITO3胜野，keiko水梨，东重文tanak。