无根根霉脂肪酶的菌株筛选及产酶条件设置.doc

无根根霉脂肪酶的菌株筛选及产酶条件设23???42?52001??6?uA?A?A??p???w???p??????JournalofDalianMedicalUniversityA?VoL23,No・2A?Jun.2001?????s??:l 000-5676(2001)02-0150-03???<?<?r?3?????????H???r???????????????????<,??A???2,???e?13(ll,?????p??A? 116027;21 ,?????p??A?l 16600;31 ,?????p??A?ll 6022)????:????:???r???????H????????????????????A3????:???8?????H???&It;?<?r(RhizopusarrhizusFischer-29)?O?T?????????3???????w??A3???????3?????????T???????: ??pH710,??????????l%,32(0A3?G??????48h,?????????????w34198u/ml,?F???????a?C??????,???????8??DHAA2EPA???%???E??????10%?????A40%,?m?????7???????Y??????????,?3 ?u???.?s?5??A3???/??:???5???3????;RhizopusarrhizusFischer?8???J????:R392A?A?A????/?o???2:AA?A??3????(Lipase,EC3・ 1 ・ 1 ・3,?y???3?C????)?m?J???3??????A3?F???f?????????5???8???????F,???m?????????????/?C????,?c???F?3???????s?C?f???p????????(?3)7U?C?????n?C??????,?4???????????H???<?<?r(RhizopusarrhizusFischer-29)?????F???i????????????????????????,?T???3????????? 1 ?J???????????5?M???????r?????0A31 A???????????111A????H????100ml?????????j?8?%015%?B????,pH710, 32(0,?G??????48h(?r??A2????)?s24h(????)A3 112A??3?????w??????[2]???FWatanabeN?????????????3???<??A32A?????21 lA??3???????5???????r???f??⑶??????25?H?r??A217• JL JL • • • • • •丿 ・JL・ • • •人• • • ••••••••••，••• JL・• • X / • JL JL • • • • • • JL JL • • • JL J，• X V?r(RhizopusarrhizusFischer-29)?????????3????????lA??f?l???????H???3?????w?????????????U??A3????????1A3212A????<?<?r??????????????21211 A?????????????????????:?E??2????,48h??,???????3?????w??????,?????5??????????A3??A??f?l?????????????????3?????w?????????:??????????(????????3(0,5??????????, 15%?B????,?m?G?????(,?:?????])2:20ml0140/minpH,48h?D???????????????5???5A321212A??f?l???3??????????????????:?f?l???3?f?l?????????????????w????????????3A3?E??3????,????l%?????]???????????????5?3???????w????????26141 u/ml,?m?????B?????Y?????????w????! 19?f(P<0105),?????????T?????????????F????????????A3?f?l???3???l???????????????????b???F??????????l?E?? 1 ????,????Tween-80?Y???????????b999P999T 979/9^97919979 a 29B9999?9?a?9 ?f9999?3???????????b???F???EA3??3A??Pl???3?f?l???????????????????w????????(?????????l?n,????????48h)??lA??f?l???3???l???????????????????b???F?????????????3?9?????B?????????q??Tween-80?f?????3??????0?1139041382414882137821378214882???:????gll0110110110110110???:????0?1080090010001100780Vg0?l 139041302413982127821268214100?(???D???g-29-68-29-78-l 7?9??(02630263026KO2111891126111671114311287XO2-341481-85168-331843-891154-211879A?A???:????????7U?p?U???b?](Vg7U???????8?U????????A2Vf7U???????8???????????T??)??4A??f?l?????????????????3?????w?????????:??????????(?????????l?n) 21213A??????9??????????????:?E??4????,20(0 ???????????w????????25(0,?m?E?????9??????,• • • • w4 • A •丿• • • • • • • • • • • • A A A • Wfl • • • JL •丿• •???G??,?????????5?s?????5???.????,???r?9???3 ???????5?M,???]?9?????????.???????8?????????5???F???????FA2?b???Q???A?C??A3?E??????,???? ?H?????3?????9???T3(0A3?E?n???????????a( 1,%????,3(O?G??????8)?????Y???????????w???T318,?D?????y?s???????r?0?????3(P<0105)A3Rh.ar.Fischer-29?????????????? ???[?????G,???????????C?????O?p????????????9? 79799997979Y959p9U79999.9n ?9?9S A73A??????3?????????5???A???.?h???????????],OtaY ??TjY?Q???6???T?J?3?????F?s?3?????????????????0???????3????????????,?F???????<?< ?r??,?f???????????3?Y???????w?????????Tl 151?42?5?????<?Q:???<?<?r?3?????????H???r??????????????2pH70124h49u/mlsuisaka2925A?Rh.ar.Fischer-29?????????????????[?????G(???????????C?????O?p??????????????,???????????Y?5?F?h?????:?nA3) u/ml,?????5???????b?E,???????3??????????????• • JL JL JL • • • JL • • 4 ••••••• LZ • • • ▼▼?a??,???m???3?C?????a?8???O???.?3?????v???w?????F??????,?.?????????????eA3?K?????????????8??99?9?9??9pD ?a99?9?9??U???????.???M?.?????u???v?????????]????A3A?A??F???????8?????????D,???s?3?????????????p?p????,?F?????Y?n,?e?????F???3?????C??????,??????? Y?%??DHAA2EPA?E?????? 10%?????A40%A3Tween-80?m?????????w?p?k,?????D?3???????M????,???????.?i?p?<??,???????t???????s,???1???C??????????????????????,?????????5?????u?M,?????T???????b???F?p?p???TA3?????r??9999999T9999999M9p)99999p9rp919999999 999999?0A3?K???????H?????p?U?????f?5?i??,?q???F????A2?s??A2?s?????Q???????????.?M?M???s???????????FA3[1] ?7?e?X.???]?k???.[M].?t??:???????I?0,1984.655-668・[2] ???????8?(,?????Q?H??lrhizopusdigosporus??A?????????A?????[J].???4???????.?.??,1989.36(6):43.[3] RollofJ,HedstromSA,Nilsson-EhleP,etal.Purificationandcharacter2 izationofalipasefromstaphylococcusaureus[J].BiochimBiophysActa, 1987,921:364-369・StudyonthesieveandoptimalcultureconditionsofRhizopusarrhizusFischerLipase-29SUNHong-dan1,HUJu,YAOMin-chang3(l.DepartmentofClinicalHemotology,DalianMedicalUniversity,Dalian 116027,China;2. hedicalcollegtofDalianuniversityDalian 116600,china3. 。