202X年牙周病与免疫
30页1、The oral cavity harbors more than 700 bacterial species,coexisting with probably millions of viral genomic copies.口腔定居的细菌有口腔定居的细菌有700多种多种与数百万病毒基因组复制与数百万病毒基因组复制(fzh)物共存物共存(A Tsuo Amano,2010,Periodontology 2000)第二页,共三十一页。Oral bacteria 口腔中的细菌口腔中的细菌:-Free to saliva 在唾液在唾液(tuy)中中 -Attachment in biofilm 附着在生物膜附着在生物膜 Between closely relatedSaliva as a carrier,is conducive to horizontal and vertical transmission of bacteria 第三页,共三十一页。In United States,75 per cent of adult populations suffer from periodon
2、tal disease.美国占美国占75%成年人群体不同成年人群体不同(b tn)程度患有牙周病程度患有牙周病1/3 children of 611years old,and 2/3 of adolescent population have some degree of periodontal problems.1/3的的611岁儿童,岁儿童,2/3青春期人群也有某种程度的牙周问青春期人群也有某种程度的牙周问题题第四页,共三十一页。Over 200 kinds of bacterial form dental plaque,adhere to the surface of the teeth and gums,and cause periodontal infections.牙周感染牙周感染(gnrn)细菌达细菌达200余种余种Periodontitis Gingivitis bleeding,Deep gingival Pocket 3mm Periodontitis bleeding,swelling,initial bone loss,Deep periodontal Pock
3、et=5mm major stage bleeding,swelling,major bone loss,Deep periodontal Pocket 6mm第五页,共三十一页。Periodontal Pocket:a unique micro-environment牙周袋是独特的微环境牙周袋是独特的微环境escape the physical separation force 逃逸物理分离力逃逸物理分离力hard tissue surface 不剥脱更新的硬组织表面不剥脱更新的硬组织表面junctional epithelialization,basically not differentiation有结合上皮,有结合上皮,基本上不分化基本上不分化(fnhu)non-keratinizing epithelium 无角化无角化facultative anaerobic anaerobic 兼性厌氧兼性厌氧厌氧厌氧nutrition,humidity,pH,Eh 营养、湿度、营养、湿度、pH、Eh第六页,共三十一页。第七页,共三十一页。龈下细菌龈下细菌(xjn)附着和聚集模式图附着和
4、聚集模式图RootsurfacePellicleP.gP.gP.gP.gP.g第八页,共三十一页。Subgingival biofilm formation龈下生物膜形成龈下生物膜形成 Initial colonization早期早期(zoq)定居者定居者:actinomyces,streptococci succeeded colonization 微生物交替后:微生物交替后:periodontal pathogens 致病微生物定植致病微生物定植 Pg,AA,forsythia,T.denticole Fusobacterium nucleatum第九页,共三十一页。第十页,共三十一页。1996 confirmed three bacteria for periodontal pathogens:Actinobacillus actinomycetemcomitans(AA)Tannerella forsythia(TF)Porphyromonas gingivalis(Pg)1996,牙周致病菌牙周致病菌 AA,TF,PgRecent views change,pathogeni
5、c capacity of AA and other culturable or non-culturable bacteria should be confirmed.近期近期(jn q):AA待证实待证实第十一页,共三十一页。50%oral microbial cannot be cultivated and can be identified through extra methods:口腔中有口腔中有50%微生物不能培养,可通过下述方法微生物不能培养,可通过下述方法(fngf)鉴定:鉴定:quantitative RT-PCR 定量定量PCRphylogenetic 16s-rRNA gene clone library analysis 用基因组或用基因组或16s rRNA探针探针checkerboard hybridization 棋盘杂交棋盘杂交high-throughput fingerprint technology 高通量指纹技术高通量指纹技术pyrophosphate sequencing 454技术(焦磷酸盐测序)技术(焦磷酸盐测序)metagenome tec
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