2019年最新-Identifying-Proteins-with-Tandem-Mass-Spectrometry---Lectures:识别和串联质谱蛋白质-讲座-
83页1、Interpreting MS/MS Proteomics Results,Brian C. Searle Proteome Software Inc. Portland, Oregon USA Brian.SearleProteomeSoftware NPC Progress Meeting (February 2nd, 2019),The first thing I should say is that none of the material presented is original research done at Proteome Software,but we do strive to make the tools presented here available in our software product Scaffold. With that caveat aside,Illustrated by Toni Boudreault,Organization,This is an foremost an introduction so were first going
2、 to talk about,Then were going to talk about the motivations behind the development of the first really useful bioinformatics technique in our field, SEQUEST.,This technique has been extended by two other tools called X! Tandem and Mascot.,Were also going to talk about how these programs differ,and how we can use that to our advantage by considering them simultaneously using probabilities.,Identify,SEQUEST,X! Tandem/Mascot,Differ Combine,how you go about identifying proteins with tandem mass spe
3、ctrometry in the first place,So, this is proteomics, so were going to use tandem mass spectrometry to identify proteins- hopefully many of them, and hopefully very quickly.,A,A,I,K,G,K,I,D,V,C,I,V,L,L,Q,H,K,A,E,P,T,I,R,N,T,D,G,R,T,A,Start with a protein,And to use this technique you generally have to lyse the protein into peptides about 8 to 20 amino acids in length and,A,A,I,K,G,K,I,D,V,C,I,V,L,L,Q,H,K,A,E,P,T,I,R,N,T,D,G,R,T,A,Cut with an enzyme,A,A,I,K,G,K,I,D,V,C,I,V,L,L,Q,H,K,A,E,P,T,I,R,N,
4、T,D,G,R,T,A,Select a peptide,Look at each peptide individually.,We select the peptide by mass using the first half of the tandem mass spectrometer,A,E,P,T,I,R,H2O,Impart energy in collision cell,The mass spectrometer imparts energy into the peptide causing it to fragment at the peptide bonds between amino acids.,M/z,Intensity,A,E,P,A,A,E,A,E,P,T,72.0,201.1,298.1,399.2,Measure mass of daughter ions,The masses of these fragment ions is recorded using the second mass spectrometer.,M/z,Intensity,A,E
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