截鼽汤对过敏性鼻炎大鼠模型鼻粘膜icam-1表达及血清il-5含量的影响
中文摘要目的 观察截鼽汤对实验性过敏性鼻炎大鼠模型鼻粘膜 ICAM-1 的表达及血清 IL5 含量的影响,进一步探讨截鼽汤对过敏性鼻炎的治疗机理,并为今后的临床应用提供依据。方法 采用卵清蛋白为致敏原,对大鼠进行实验性变应性鼻炎造模。60 只老鼠随机分为 6 组:模型组、空白对照组、西药治疗组和中药大、中、小剂量治疗组。造模成功后,分别用截鼽汤和扑尔敏灌胃治疗,观察其搔鼻动作,喷嚏、鼻溢液及觅食行为。处死大鼠后,采用免疫组化染色,测定 ICAM-1 的表达,检测血清中 IL5 的含量。结果 1.截鼽汤和扑尔敏均可改善过敏性鼻炎大鼠的症状。截鼽汤各剂量组及扑尔敏西药对照组与模型组比较,均有显著性差异,具有统计学意义(P0.05)。截鼽汤各剂量组与扑尔敏西药对照组比较,无显著性差异,无统计学意义(P0.05)。2.截鼽汤各剂量组及西药扑尔敏组对鼻粘膜 ICAM-1 表达的影响:用药各组 ICAM-1 的表达均低于模型组,其差异有统计学意义(P0.01);截鼽汤各剂量组阳性细胞所占面积均低于西药扑尔敏组,有显著性差异,具有统计学意义(P0.05);而截鼽汤大、中、小各剂量组之间的治疗效果差异不明显,无统计学意义(P0.05)。3.截鼽汤各剂量组及西药扑尔敏组对 AR 大鼠血清中 IL5 含量的影响:用药各组与模型组比较,均有显著性差异,有统计学意义(P0.01);中药高剂量组与中药中、低剂量组比较,有显著性差异,有统计学意义(P0.01);中药高剂量组与西药扑尔敏组治疗效果无显著差异,没有统计学意义(P0.05)。4.截鼽汤能改善过敏性鼻炎大鼠鼻粘膜病理反应。减轻炎症症状。结论 截鼽汤能够降低过敏性鼻炎大鼠模型的血清 IL5 含量及鼻粘膜 ICAM-1的表达,并改善其鼻部症状,减少鼻溢液的分泌及 AR 大鼠喷嚏、搔鼻动作的次数,从而达到治疗的目的。关键词 截鼽汤;过敏性鼻炎;血清 IL5;ICAM-1The Effect of JieQiutang on ICAM-1 to express and IL5in serum of Allergic RhinitisAbstractObjective:to observe the sectional JieQiutang on experimental allergicrhinitis nasal mucosa model of rats ICAM-1 to express and serum IL-5 levelsof influence,further discussion section JieQiutang to allergic rhinitistherapeutic mechanism,and for future clinical application provides the basis.Methods:usingeggalbuminforsensitizationoriginal,ratstoexperimental allergic rhinitis made moulds.60 mouse randomly divided into sixgroups:model group and control group,western medicine therapy group oftraditional Chinese medicine and the large.medium and small dose treatmentgroup.Build mode after the success,respectively for JieQiutang and flappingdarfur sensitive irrigation stomach treatment,observe its scratching noseaction,sneezing,nasal nipple discharge and foraging behavior.Rats afterdeath,usingimmunohistochemicalstains,determinationICAM-1expression,detection of serum IL-5 content.Results:1.JieQiutang and Darfur sensitive all can improve allergicrhinitissymptomsofrats.JieQiutangeachdosegroupofwesternmedicine,sensitive and flapping group compared with model control group,allhavesignificantdifferencearestatisticallysignificant(P0.05).JieQiutang with each dose group of western medicine rcpmaj flapping 'ssensitivity,no significant difference was statistically significant(P 0.05).2.JieQiutang each dose group of JieQiutang and western medicine groupto save darfur sensitive nasal mucosal ICAM-1 express influend:drug ICAM-1each expression are below model group,the difference was statisticallysignificant(P0.01),JieQiutang each dose group of positive cells occupiedarea were lower than of western medicine flapping's sensitive group,wassignificant difference,a statistically significant(P0.05).And JieQiutanglarge,medium and small between each dose group of the therapeutic effect ofno obvious difference,not statistically significant(P0.05).3.JieQiutangeach dose group and western medicine group to save darfur sensitive AR ratsserum IL-5 levels influence:each group compared with model medication,allhave significant difference was statistically significant(P0.01),Chinesemedicine high-dosage groups and Chinese medium medium,low dose group ofcomparison,have significant difference was statistically significant(P0.01).High dose group of TCM and western medicine treatment group'ssusceptibilityflappingeffectnosignificantdifferencewasnotstatistically significant(P 0.05).4.JieQiutang can improve allergicrhinitis nasal mucosa rats pathological reaction.Reduces inflammationsymptoms.Conclusion:JieQiutang can reduce allergic rat model of serum IL-5contentand nasal mucosal ICAM-1 expression,and improve its nasal symptoms,reducingnasal nipple discharge secretion and AR rats sneezing,scratching nose actionfrequency.So as to achieve the oblective of treatment.Keywords:JieQiutang,Allergic rhinitis,SerumIL-5,ICAM-1Author: Wang XiaoqianTutor : Zhang Xiong陕西中医学院学位论文独创性声明及使用授权声明学位论文独创性声明本人郑重声明: 所呈交的学位论文,是个人在导师的指导下,独立进行研究工作所取得的成果,无抄袭及编造行为。除文中已经特别加以注明引用的内容外,本论文不含任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重要贡献的个人和集体,均已在文中以明确方式标明并致谢。本人完全意识到本声明的法律结果并由本人所承担的法律责任。学位论文作者签名日 期:年月日关于学位论文使用授权的声明陕西中医学院有权保留使用本人学位论文,同意学院按规定向国家有关部门机构送交论文的复印件和电子版,允许被查阅和借阅。本人授权陕西中医学院可以将本学位论文的全部或部分内容编入有关数据库进行检索,可以采用影印、缩印或其他复印手段保存和汇编本学位论文。可以公布(包括刊登)论文的全部或部分内容。(保密论文在解密后应遵守此规定)论文作者签名日 期:论文导师签名日 期:年年月月日日截鼽汤对过敏性鼻炎大鼠模型鼻粘膜ICAM1表达及血清IL5含量的影响引 言过敏性鼻炎(Allergic RhinitisAR)亦称变态反应性鼻炎,相当于祖国医学的“鼻鼽”或称“鼽涕”,是鼻粘膜感受特异性过敏原的刺激,鼻粘膜自主神经系统中的交感神经平衡失调而导致的疾病1,以阵发性喷嚏、鼻塞、鼻痒、分泌大量清水样鼻涕、鼻粘膜水肿为主要病症表现,具有反复发作、迁延难愈的特点。本病的主要发病机制属 I 型变态反应,但是与细胞因子、细胞间粘附分子-1(Intercellular Adhesion Molecule-1,ICAM-1)及部分神经肽的相互作用密切相关2。当特异性抗原进入特应性个体后,机体内产生相应的免疫球蛋白 E(IgE)抗体(亦称反应素),并附着于介质细胞(肥大细胞、嗜酸性粒细胞)的表面,此时机体即处于致敏状态。当相同的抗原再次侵入该机体时,则此抗原与介质细胞表面的 IgE 发生“桥联”,并激发细胞膜产生一系列的生化变化,破裂并脱颗粒。从被排除的颗粒中和细胞内释放的生物活性介质,如组胺(histamine)、白三烯(LT)、缓激肽(bradykinin)等,这些介质能引起毛细血管扩张、血管通透性增加、平滑肌收缩和腺体分泌增多等病理变化,机体处于发敏状态,临床上则表现为喷嚏、清涕、鼻塞、鼻痒等典型症状3。过敏性鼻炎是一种常见病、多发病。随着工业化程度的发展及生态环境的恶化,过敏性鼻炎的患病率在全球有增长的趋势,发达国家成人发病率已达 1020,甚至高达 304。我国每年大约有 2000 万人患病,发病率 37.745。过敏性鼻炎的并发症往往多见,主要有支气管哮喘、过敏性鼻窦炎、过敏性中耳炎、过敏性咽喉炎等;此外,过敏性鼻炎常常合并结膜炎,鼻息肉,慢性阻塞性肺病、荨麻疹或湿疹等,严重影响人们的正常工作学习生活。所以,过敏性鼻炎