生物专业ppt

Pesticidal and pest repellency activities of a plant derived triterpenoid2,3,21,23,28-pentahydroxyl 12-oleanene against Tribolium castaneum.,Reporter:XXX Date：2018.05.27,Laportea crenulata, Tribolium castaneum 2,3,21,23,28-penta hydroxyl 12-oleanene, Botanical pesticide, Botanical pest repellent.,Keywords,Contents,01,02,03,Background,Methods,Results,Tribolium castaneum is a major pest of stored grain-based products, and cause severe damage to cereal grains throughout the world. Synthetic agents has led to environmental disturbances, lethal effects on nontarget organisms and direct toxicity to users. The roots of the plant have stimulant, stomachic and diuretic properties ,suggest the presence of biologically active substances in roots of the plant.,Background,Plant materials Isolation of 2,3,21,23,28-penta hydroxyl 12-oleanane Collection and maintenance of pest Screening for insecticidal activity Insect repellency test,Methods,Results,These data suggest that the triterpenoid 2,3,21,23,28-penta hydroxyl 12-oleanene possess both pesticidal and pest repellency activities against T. castaneum and can be used in controlling the pest of grain-based products.,Conclusion,诱集植物香根草对二化螟幼虫致死的作用机制,一、背景,香根草能够有效诱集水稻害虫二化螟雌成虫在其上产卵，但孵化出的幼虫在香根草上不能完成生活史。香根草作为诱集植物防治稻螟已成为水稻害虫绿色防控的重要措施之一，具有很大的开发潜力。,二、方法,（1）香根草活性物质对二化螟幼虫的生物活性测定 （2）水稻和香根草中营养物质含量测定 （3）取食水稻和香根草后二化螟幼虫体内解毒酶活性测定 （4）取食水稻和香根草后二化螟幼虫体内消化酶活性测定,三、结果与分析,图 1 香根草提取物对二化螟 3 龄幼虫的生物活性,（1）香根草活性物质对二化螟幼虫的生物活性测定,图 2 香根草石油醚萃取物中不同组分对二化螟 3 龄幼虫的生物活性,三、结果与分析,（2）水稻和香根草中营养物质含量测定,图 3 水稻和香根草中的营养物质含量,表 1 水稻和香根草茎秆中氨基酸种类和含量,三、结果与分析,（3）取食水稻和香根草后二化螟幼虫体内解毒酶活性测定,图 4 二化螟 3 龄幼虫取食水稻和香根草后体内解毒酶活性变化,三、结果与分析,（4）取食水稻和香根草后二化螟幼虫体内消化酶活性测定,图 5 二化螟 3 龄幼虫取食水稻和香根草后体内消化酶活性变化,四、结论,香根草中有毒物质抑制了二化螟体内 Car E 和细胞色素 P450 酶的活性；香根草内营养物质缺失，单宁含量高，从而影响二化螟体内消化酶活性的变化，这些可能是二化螟幼虫取食香根草后致死的两大重要因素。研究结果可为开发以香根草为基础的稻螟绿色防控新技术提供理论依据。,